目的:建立广藿香紫苏叶挥发油微乳中百秋李醇的GC含量测定方法。方法:采用挥发油提取器从微乳中提取挥发性成分,用GC法测定挥发油微乳中百秋李醇的含量。色谱条件:HP.5色谱柱;柱温采用程序升温;气化温度为270℃;检测器温度为280℃;分流比为20:1;流速为1mL·min^-1。结果:表明百秋李醇在0.4064-4.8768弘g范围内呈良好的线性关系,回归方程为y=0.58904X-0.0214,r=0.9998,平均回收率为97.10%(n=5)。结论:方法准确可靠,重复性好。可用于挥发油微乳中百秋李醇的含量测定。
Objective:To determine the content of patchouli alcohol in microemulsion of volatile oils of Cablin Patchouli Herb and Perilla Leaf by GC. Methods: HP-5 column was used. Column temperature was controlled by a temperature programm. The injector temperature was 270 ℃ and the detector temperature was 280 ℃. The flow rate of carrier gas was 1 mL·min^- 1 . Results : A good linearity of patchouli alcohol was found over the injection a mounts of 0.406 4μg - 4.876 8μg. The regression equation was Y = 0.589 0X - 0.021 4( r = 0.999 8) . The average recovery was 97.10% with a RSD of 1.23%. Conclusion: The method is simple and accurate and can be used as a quality control method for the microemulsion of volatile oil of Cablin Patchouli Herb and Perilla Leaf.