中文摘要：

旨在研究化学改性的甘蔗渣作为固定化载体对丙酮丁醇梭菌ClostridiumacetobutylicumxYl6发酵制备生物丁醇的影响。首先利用不同浓度的聚乙烯亚胺（PEI）和1g／L戊二醛（GA）对甘蔗渣表面进行化学改性，增强甘蔗渣对ClostridiumacetobutylicumXYl6的附载能力。经4g／L聚乙烯亚胺和lg／L戊二醛改性的甘蔗渣（添加量10g／L1应用到固定化批次发酵中，发酵36h后丁醇和总溶剂浓度最高，分别达到了12．24g／L和21．67g／L，同时溶剂的生产速率达到0．60g／（L·h），生产速率比游离细胞和未改性甘蔗渣固定化细胞分批发酵分别提高了130．8％和66．7％。在此基础上对改性甘蔗渣固定化的细胞进行6次重复批次发酵，丁醇和总溶剂的产量稳定，溶剂生产速率逐渐提高至0．83g／（L．h），同时转化率也提高至0．42g/g。

英文摘要：

Sugarcane bagasse modified by polyethylenimine （PEI） and glutaraldehyde （GA） was used as a carrier to immobilize Clostridium acetobutylicurn XY16 in the process of butanol production. The effects of chemically modified sugarcane bagasse on batch and repeat-batch fermentations were investigated. Batch fermentation was conducted with anaddition of 10 g/L modified sugarcane bagasse and 60 g/L glucose, resulting in a high solvent concentration of 21.67 g/Land productivity of 0.60 g/（L-h） with the treatment of 4 g/L PEI and 1 g/L GA. Compared to the fermentations by free cellsand immobilized cells fibrous-bed bioreactor maximum productivity on unmodified sugarcane bagasse, the productivity increased 130.8% and 66.7%, respectively. The also maintained a stable butanol production during repeat-batch fermentations, achieving a of 0.83 g/（L.h） with a high yield of 0.42 g/g.