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维药毛菊苣降糖有效部位的纯化工艺
  • ISSN号：1001-1528
  • 期刊名称：中成药
  • 时间：2012
  • 页码：2322-2325
  • 分类：R284.2[医药卫生—中药学;医药卫生—中医学]
  • 作者机构：[1]中国科学院新疆理化技术研究所新疆特有药用资源利用国家重点实验室培育基地,新疆乌鲁木齐830011, [2]中国科学院研究生院,北京100049
  • 相关基金：新疆少数民族医药现代化基金项目（200733146-4）; 国家杰出青年科学基金（30925045）
  • 相关项目：民族药学
作者： 张尧|信学雷|阿吉艾克拜尔·艾萨|
关键词： 毛菊苣, 大孔吸附树脂, 纯化工艺, Cichorium glandulolsum, macroporous resin, purification
中文摘要：

目的利用大孔吸附树脂探索毛菊苣降糖有效部位的最佳纯化工艺。方法以静态吸附法考查9种大孔树脂（HPD100、HPD300、HPD450、HPD600、HPD750、X-5、ADS-17、D4020、D140）对有效部位的吸附和解吸附性能,结合纯化后有效部位对糖尿病及其并发症相关的蛋白酪氨酸磷酸酶1B（PTP-1B）、醛糖还原酶（AR）的抑制率及ABTS抗氧化能力的评价,通过综合评分选出纯化效果最佳的树脂进行进一步研究,确定出最佳纯化工艺。结果确定HPD100大孔树脂为最佳纯化树脂;最佳上样药液质量浓度为75 mg/mL;上样量6 BV;吸附速率为3 BV/h。洗脱前先用水洗（5 BV/h）4 BV除杂,再用60%乙醇以3 BV/h速度洗脱5 BV获得有效部位。结论经HPD100大孔树脂处理后的毛菊苣降糖有效部位对PTP-1B抑制活性可达到纯化前的10倍。该方法简单易行,对提高活性有明显效果,并对工业化生产有指导意义。

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期刊信息
  • 《中成药》
  • 北大核心期刊（2011版）
  • 主管单位:
  • 主办单位:国家食品药品监督管理局 信息中心中成药信息站
  • 主编：陶建生
  • 地址：上海市黄浦区福州路（近江西中路）107号206室
  • 邮编：200002
  • 邮箱：zcy.med@foxmail.com
  • 电话：021-63213275
  • 国际标准刊号：ISSN：1001-1528
  • 国内统一刊号：ISSN：31-1368/R
  • 邮发代号:4-249
  • 获奖情况:
  • 首届中国学术期刊《CAJ－CD规范》优秀期刊
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）,中国北大核心期刊（2000版）
  • 被引量:62184