利用硅胶、Sephadex LH-20凝胶柱层析和高效液相色谱(HPLC)等方法对采自湛江海百合共附生真菌Alternaria brassicae 93的次级代谢产物进行分离纯化,根据化合物的理化性质、波谱数据以及与文献数据对照的方法确定化合物的结构。从海百合共生真菌Alternaria brassicae 93的次级代谢产物中分离得到5个化合物,分别鉴定为clavatol(1)、3R,14S-ochratoxin A(2)、3R,14S-ochratoxin B(3)、chaetoquadrin F(4)、ent-cycloechinulin(5)。化合物1,2,3,4,5,均为首次从海洋真菌中得到。
Secondary metabolities of the crinoid epipsymbiosis fungus Altermaria brassicae 93 from South China Sea were studied. The metabolities of Alternaria brassicae 93 were isolated by silica gel column chromatography,Sephadex LH- 20 gel column chromatography and HPLC,the structures of compounds were eventually identified and confirmed on the basis of physicochemical properties,spectral data and compared with the published spectral information. Five compounds were isolated from marine fungi for the first time and their structures were identified as clavatol(1),3R,14S-ochratoxin A(2),3R,14 Sochratoxin B(3),chaetoquadrin F(4) and ent-cycloechinulin(5).