中文摘要：

以N-羟基琥珀酰亚胺（NHS）和1-（3-二甲氨基丙基）-3-乙基碳二亚胺盐酸盐（EDC·HCl）为催化剂,将制备的双羧基聚乙二醇（COOH-PEGCOOH）和部分巯基化聚乙烯亚胺（PEI-SHx ）先后接枝于壳聚糖分子,得到巯基化壳聚糖衍生物（CS-PEGPEI）.利用傅立叶红外光谱仪（FT-IR）和核磁共振仪（1H NMR）对其结构进行表征;通过透析法制备自聚集纳米粒,透射电子显微镜（TEM）和动态激光粒度分析仪（DLLS）分析测试自聚集纳米粒显示,自聚集纳米粒为球状纳米胶束,该纳米球有望成为纳米药物及基因的载体.

英文摘要：

Thiolated chitosan derivative （CS-PEG-PEI） was synthesized by grafting double carboxy-terminated PEG and partly thiolated polyethylenimine （PEI-SHx） onto chitosan with NHS/EDC as catalyst. The chemical structure of polymer was characterized by the Fourier transform infrared spectroscopy（FT-IR） and proton nu- clear magnetic resonance（1 H NMR）, the self-assembled nanoparticles was prepared by probe sonication-dialysis method. The measurements of transmission electron microscope（TEM） and dynamic light scattering（DLS） in- dicated that the self-aggregates were nanoparticles. This novel nanoparticle system was promising to be a good drug carrier and gene carrier.