中文摘要：

本研究以高山离子芥试管苗为材料,研究了渗透调节物质和保护酶系对其低温胁迫的响应机制。结果表明：渗透调节物质脯氨酸、可溶性糖、可溶性蛋白分别以各自不同的响应方式在冷冻胁迫下增强高山离子芥的抗寒性。保护酶系中,超氧化物歧化酶（SOD）和过氧化物酶（POD）在减轻低温胁迫下高山离子芥试管苗膜脂过氧化伤害作用中协同互补作用；过氧化氢酶（CAT）和抗坏血酸过氧化酶（APX）在低温胁迫下高山离子芥试管苗清除过氧化氢机制中发挥主要作用,且CAT对低温胁迫的响应强度远高于 APX,说明保护酶系在抵制低温胁迫和保护高山离子芥免受低温伤害方面具有重要的生物学功能。

英文摘要：

Taking Chorispora bungeana plantlets in vitro as material,the mechanism of osmotica and protective enzymes response to the low temperature stress were studied.The results showed that pro-line,soluble sugar and soluble protein involve in the strengthen Chorisporabungeana cold resistance.In the protective enzyme system,SOD and POD have significant complementary effect on mitigating the in-jury of membrane lipid peroxidation of Chorispora bungeana;CAT and APX play major roles in the mechanism of removal of hydrogen peroxide,and CAT responding to low temperature stress intensity is much higher than APX.All of these indicated that protective enzyme system in resistance to low temper-ature stress and protecting Chorispora Bungeana from low temperature damage had important biological functions.