中文摘要：

目的探讨利用Nodal基因表达预测肝癌转移复发的可行性。方法通过RNA干扰技术对人肝癌细胞Nodal基因表达的沉默，观察对肝癌细胞生物学行为及血管生成拟态形成的影响。设计4条Nodal基因特异干扰序列，通过质粒载体转染人肝癌细胞株SMMC-7721，分别以实时荧光定量PCR和Westernblot检测Nodal基因和蛋白的表达水平。观察Nodal基因干扰对肝癌细胞增殖、凋亡、侵袭、迁移能力及血管生成拟态的影响。结果RNA干扰明显抑制了肝癌细胞Nodal基因的表达。在增殖实验第4、5、6天，干扰组的增殖率明显低于对照组（分别F=17098．922，18135．107，32641．075，均P〈0．05）。转染48h后，干扰组凋亡率明显高于对照组（F=1136．452，P〈0．05）。在侵袭和迁移试验中，干扰组穿过transwell小室的细胞数明显低于对照组（分别F=83．6，1126．857，均P〈0．05）；转染24h和48h后，干扰组均未形成血管生成拟态，明显区别于对照组。结论干扰Nodal基因的表达，可明显抑制肝癌细胞的生物学行为及血管生成拟态的形成。

英文摘要：

Objective Toevaluate the role of nodal gene modulating malignancy of a hepatocellular carcinoma cell lines SMMC7721. Methods To silence the expression of nodal gene in human hepatocellular carcinoma cells by RNA interference （ RNAi）, and to observe the effect on cells biological behaviour and vasculogenic mimicry. 4 expression vectors of nodal gene targeting small interference RNA were constructed and transfected into SMMC-7721 cells. Real-time quantitive PCR and Western blot were used to examine nodal gene expression. The effects of nodal gene RNA interference on proliferation, apoptosis, infestation, migration and vasculogenic mimicry of SMMC-7721 were studed. Results The expression of nodal gene was suppressed in SMMC-7721 cells by RNA interference. In the first 4, 5, 6 days of proliferation experiment, the proliferation of interference group was significantly lower than the control group（separately F = 17 098. 922, 18 135. 107,32 641. 075, all P 〈 0. 05） ; 48 h after transfeetion, the apoptosis rate of interference group was significantly higher than the control group （F = 1136. 452, P 〈 0. 05）; In the infestation and migration experiments, the cells through the transwell chamber in the interference group were less than the control group（ separately F = 83.6,1126. 857, all P 〈 0. 05 ） ; 24 h and 48 h after transfection, the vasculogenic mimicry in the interference group did not form which was significantly different from the control group. Conclusions Interfering the expression of nodal gene inhibits the malignant biological behaviour and the formation of vasculogenic mimicry in human hepatocellular carcinoma cells.