中文摘要：

目的探讨外源性转化生长因子-β3（TGF-β3）对大鼠肝星状细胞（HSC）分泌内源性TGF-β3及细胞增殖的影响。方法取大鼠HSC接种于12孔板，一组用不同浓度（0．08、0．4、2、10和50ng／ml）的外源性TGF-β3干预2h后收集上清液，另一组用10ng／ml外源性TGF-β3干预后在不同时间点（15min、30min、1h、2h、4h、8h、βh）收集上清液，应用ELISA法检测TGF-β3含量。另取HSC接种于96孔板，一组用不同浓度（0．001、0．005、0．02、0．08、0．32、1．25、5、20、100、500ng／ml）的外源性TGF-β3干预24h，另一组用5ng／ml外源性TGF-β3干预24和48h，用噻唑蓝（MTT）法检测细胞增殖情况。倒置显微镜下观察空白对照组和外源性TGF-β3干预组HSC的大体形态。结果当外源性TGF-β3浓度达2ng／ml时可明显促进内源性TGF-β3表达，随后内源性TGF-β3的表达量呈外源性TGF-β3浓度依赖性增长（F=210．168，P=0．00），该作用3h时达高峰[（0．845土o．028）ng／ml比（0．026±0．022）ng／ml]。外源性TGF-β3浓度≥0．32ng／ml时对HSC增殖有较弱的促进作用，但无剂量依赖关系（F=0．68，P=0．57）。经TGF-β3干预的HSC镜下形态与空白对照组相比未出现明显变化。结论外源性TGF-β3可促进HSC中内源性TGF-β3的表达，还可促进HSC增殖。外源性TOF-β3干预对HSC细胞大体形态无明显影响。

英文摘要：

Objective To investigate the effects of exogenous transforming growth factor-β3 （TGF-β3） on the expression of endogenous TGF-β3 and proliferation of rat hepatic stellate cells （HSC）. Methods Rat HSC cells were seeded in 24-well plates and were divided into 2 groups. One group of cells were exposed to TGF-β3 of different concentrations （0.08, 0.4, 2, 10 and 50 ng/ml） for 2 hours and then the cell culture supernatant was collected; the other group of cells were exposed to 10 ng/ml TGF-β3 arid the cell culture supernatant was collected at different time point （0.25, 0.5, 1, 2, 4, 8 and β h）. The content TGF-β3 was determined by ELISA method. Some other HSC cells were seeded in 96-well plates and divided into 2 groups. One group of cells were cultured in the presence of exogenous TGF-β3 of different concentrations （0. 001, 0. 005, 0.02, 0.08, 0.32, 1.25, 5, 20, 100, 500 ng/ml） for 24 hours and then the cell proliferation was detected; the other group of ceils were treated with 5 ng/ml TGF-β3 for 24 and 48 hours. The cell proliferation was measured by MTT method. The HSC cell morphology of control group and TGF-β3 treated group was observed under inverted microscope. Results The endogenous TGF-β3 expression of HSC cells obviously increased after exogenous TGF-β3 treated at 2 ng/ml and reached the peak at 3 hour [（0. 845±0. 028） ng/ml vs （0. 026±0. 021） ng/ml,F=210. 168,P=0. 001. Low concentrations of exogenous TGF-β3 did not affect the proliferation of HSC cells. Above 0.32 ng/ml, exogenous TGF-β3 could promote HSC proliferation. There was no dose-dependent relationship between HSC cell proliferation and the concentration of exogenous TGF-β3 （F= 0.68, P= 0.57）. Under microscope, there was no significant difference in HSC cell morphology between control group and TGF-β3 treated group. Conclusions Exogenous TGF-β3 can promote the expression of endogenous TGF-β3 in HSC cells, and can promote HSC cell proliferation. There is no obvious effect of exogenous TGF-β3 on HSC