中文摘要：

目的：研究低频超声联合微泡剂对血管内皮细胞VEC-304的作用，为超声治疗的临床应用提供实验依据。方法：将细胞分为空白对照（A）组；单纯微泡剂（B）组；单纯超声（C）组，因给予不同时间的超声辐照又分为C1组（60S）、C2组（90s）、C3组（120S）和C4组（150S）；超声联合微泡剂（D）组，因加入微泡剂后立即给予不同时间的超声辐照又分为D1组（60S）、D2组（90S）、D3组（120S）和D4组（150S）。MTT法观察细胞增殖的抑制作用，分光光度法检测细胞培养液中活性氧（ROS）和超氧化物歧化酶（SOD）活力的变化，流式细胞仪（FCM）检测细胞凋亡。结果：超声联合微泡剂能够显著抑制细胞增殖；C3、D3组培养液中ROS活力显著高于A、B两组（P〈0．01），SOD活力显著低于A、B两组（P〈0．01），且D3组效应比C3组更为明显；C3、D3组细胞凋亡率明显高于A、B组（P〈0．01），D3组高于C3组（P〈0．05）。结论：低频超声联合微泡剂可能是通过增加细胞外ROS的活力来诱导对细胞的损伤和凋亡。

英文摘要：

Objective To observe the bio-effects on VEC-304 treated with low-frequency ultrasound and micro bubble agent, and to offer the clinic application theory basis of ultrasound. Methods Cells were divided into four groups ： control （A） ; pure microbubble agent （B） ; pure ultrasound （C）, according to the exposure time , group C was subdivided into C1 （ exposure 60 s）, C2 （ exposure 90 s） , C3 （ exposure 120 s） and C4 （ exposure 150 s） ; low-frequency ultrasound combined with micro bubble agent（D） , according to the exposure time , group D was subdivided into D1 （exposure 60 s） , D2 （ exposure 90 s）, D3 （ exposure 120 s） and D4 （ exposure 150 s）. The survival rate of VEC-304 was detected by MTI＇ colormetric assay. Visible spectrophotometric was applied to determine ROS and SOD. Apoptosis was analyzed by flow cytometry after ultrasonic exposure. Results The proliferation of group D were obviously inhibited ,which was detected by MTT colormetric assay. The yield of ROS of groups C3 and D3 was higher than groups A and B, respectively. The yield of SOD of groups C3 and D3 was lower than groups A and B, respectively. The apoptosis rates were found to reach（ 11.57 ± 2.68）% in group C3 and （21.15 ± 4.68）% in group D3 by FCM. Conclusion Low-frequency ultrasound combinding with microbubble agent can induce VEC-304 injury and apoptosis,which is probably due to the adding yield of ROS in cultured liquid .