中文摘要：

通过非共价键修饰方法制备了兼具良好生物相容性和导电性的磷脂1-棕榈酰-2-油酰甘油-3-磷酸钠-石墨烯（POPG-GP）纳米复合物，并利用TEM，FT-IR，UV-vis，zeta电位等对其形貌和结构进行了表征. 由于POPG-GP纳米复合材料在水溶液中呈现出较为明显的负电性，因此，可通过静电自组装方法将辣根过氧化物酶（HRP）进一步组装到POPG-GP修饰的玻碳（POPG-GP/GC）电极上，构筑HRP/POPG-GP/GC修饰电极. 电化学实验结果表明，POPG-GP纳米复合物能够有效实现HRP与修饰电极之间的直接电子转移. 此外，固载在修饰电极表面的HRP对底物H2O2还表现出良好的电催化活性. HRP/POPG-GP/GC修饰电极对H2O2的检测线性范围为3.5～210 μmol/L，最低检出限为1.17 μmol/L（S/N=3），灵敏度为356.6 mA·cm-2·M-1，Km值为0.45 mmol/L.

英文摘要：

A novel lipid based carbonaceous nanocomposite, 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-（1＇-rac-glycerol） （sodium salt） （POPG） modified graphene （GP） （POPG-GP）, was designed and synthesized by a non-covalent method. The nanocomposite was endowed with excellent properties of the two independent components, such as the biocompatibility of POPG and the outstanding electric properties of graphene. Fourier transform infrared （FT-IR） spectra, ultraviolet-visible （UV-vis） absorption spectra, transmission electron microscopy （TEM） were utilized to characterize the structure, morphology and surface property of the as synthesized POPG-GP. It has been found that the modification of POPG on GP could not only assist the dispersion of graphene in aqueous solution, but also endow it with negatively charged, which was favorable for the further immobilization of model enzyme via self-assembly. Based on the electrostatic interaction, the positively charged horseradish peroxide （HRP） could be immobilized onto the surface of POPG-GP to form HRP/POPG-GP/GC electrode. UV-vis and FT-IR spectroscopies were used to monitor the assembly process and demonstrated that HRP had been immobilized without denaturation. The HRP/POPG-GP/GC electrode could commendably realize the direct electron transfer （DET） between electrode and redox enzyme with good electrochemical performance. Moreover, such modified electrode also showed good electrocatalytic response toward the detection of H2O2 with high sensitivity, wide linear range, excellent stability and reproducibility. The linear response range for the HRP/POPG-GP/GC was 3.5～210 μmol/L （R=0.999）. The detection limit and the sensitivity of the HRP/POPG-GP/GC electrode was calculated to be 1.17 μmol/L （S/N=3） and 356.6 mA·cm-2·M-1, respectively. The apparent Michaelis-Menten constant Km was estimated to be 0.45 mmol/L, indicating a high affinity of HRP to H2O2 on POPG-GP. The experiment results demonstrated that POPG-GP not only provided a bioc