中文摘要：

目的 探讨芪丹颗粒剂预防性治疗矽肺纤维化的作用。方法 40只Wistar大鼠分为：正常对照组、模型组、芪丹组、汉甲组4个组。除正常对照组外，其余各组采用气管注射染尘（每只鼠50mg）方法建立动物模型，芪丹组和汉甲组分别从造模后第2天灌注芪丹颗粒剂（3125mg／kg）和汉防己甲素（22mr／kg）；5个月后处死，进行肺泡灌洗，取支气管肺泡灌洗液（ bronchoalveolar lavage fluid, BALF）、肺脏、肾脏标本。称取大鼠体重、鼠肺湿重，计算大鼠肺系数；用碱水解法测定羟脯氨酸含量；用酶联免疫吸附测定法（ELISA）测定肺泡灌洗液中转化生长因子β1（TGF-131）的表达；同时对肺组织作HE染色、VG染色和网状纤维染色（gomorri），普通光镜下观察各组矽结节的细胞构成，胶原纤维及网状纤维改变；同时取肾组织作HE染色，普通光镜下观察肾脏病理改变。结果 芪丹组、汉甲组的肺系数、羟脯氨酸含量、BALF中TGF-131的表达均低于模型组，差异有统计学意义。模型组主要以Ⅲ～Ⅳ级矽结节为主，胶原纤维粗大、排列致密，网织纤维稀疏，芪丹颗粒及汉防己甲素预防治疗组主要以Ⅱ级矽结节为主，胶原纤维细小、排列疏松，网织纤维密集；汉甲组大鼠出现肾损伤，其余各组大鼠肾组织正常。结论 芪丹颗粒剂有预防和延缓矽肺纤维化的作用，安全无肾毒性。

英文摘要：

Objective To investigate the anti-fibrotic effects of Qidan granule in rats. Methods The rats were randomly divided into six experimental groups： normal group, model group, Qidan group, Tetrandrine group. All rats except normal group were treated with silicon dioxide （50 mg/rat ） by intracheal instillation to induce silicosis. Qidan group and Tetrandrine group were treated with Qidan granule（3125 mg/kg）or treated with Tetrandrine （22 mg/kg）respectively. All the rats were sacrified after 5 months. Calculate Lung/body coefficient by weighting the lung wet weight and the body weight of rats. Content of Hydroxyproline was measured by alkaline hydrolysis. The gene expression of transforming growth factor-betal was examined by using enzyme-linked immunosorbent assay （ELISA）. Paraffinembedded lung sections with HE staining,VG staining and Gomorri staining were observed under light microscope. Results In Qidan group and Telrandrine group , Lung/body coefficient and content of Hydroxyproline and expression of lransforming growth factor-betal were lower as compared with model group （ P 〈 0. 05 ）. Model group mainly showed Ⅲ～Ⅳ grade silicotic nodule, which contained thick collagen and sparse reticulum fibe; Qidan group and Tetrandrine group appeared with Ⅱ grade silicotic nodule, which contained tiny collagen and intensive reticulum fibe. Tetrandrine group showed injury of kidney, and others were normal. Conclusion Qidan granule extract should prevent and from inhibit the remarkably silicotic fibrosis in rats.