探讨一氧化氮是否参与了水杨酸(SA)诱导的采后番茄果实抗病性反应及其作用机制。以丽春绿熟期番茄果实为材料,对其进行水杨酸甲酯(MESA)处理和MeSA结合一氧化氮合酶(NOS)抑制剂L—NNA处理,检测不同处理对番茄果实抵抗病原菌侵染能力、NOS活性、NO水平以及抗病相关酶苯丙氨酸解氨酶、多酚氧化酶、几丁质酶和β-1,3.葡聚糖酶活性的影响。结果表明:MeSA处理提高了采后番茄果实抵抗病原菌侵染的能力,NOS抑制剂处理通过抑制NOS的活性和NO的水平,不同程度得削弱了抗病相关酶的活性,说明NO参与TSA诱导的采后番茄果实抗病性反应。
Salicylic acid (SA) and nitric oxide (NO) are important signaling molecules in disease resistance of plants. Previous studies have demonstrated the NO involvement in SA pathway. In order to explore whether NO involvement in defense response of SA-induced disease in postharvest tomato fruits and corresponding mechanisms, tomato fruits (Lycopersicon esculentum cv. Lichun) were treated with methyl salicylate (MESA) and nitric oxide synthase (NOS) inhibitor (L-NNA). The effect of L-NNA on the resistance to Botrytis cinerea, NOS activity, NO level and defensive enzyme activity was explored. Our results showed that MeSA treatment improved disease resistance and L-NNA treatment inhibited the activities of defensive enzymes including phenylalanine ammonialyase (PAL), chitinase (CHI),β-1,3-glucanase (GLU) and polyohenoloxidase (PPO). Therefore, NO was involved in defense response of SA-induced disease in postharvest tomato fruits.