以平菇(Pleurotus ostreatus) F5为实验材料,研究其在不同体积分数品质改良因子培养7d后,菌丝生物量的变化,以及添加体积分数0.5%品质改良因子后,不同发酵时期菌丝体中DPPH自由基和羟自由基(·OH)清除率、总抗氧化能力(T-AOC)及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)4个抗氧化相关酶活性的变化。结果表明:与对照组相比,0.5%品质改良因子处理组菌丝体干质量提高了19.31%,DPPH自由基清除率无明显变化,·OH清除率在5~7d提高了8%~11%,培养前5d菌丝体的总抗氧化能力均高于对照,在第5天的提高了32%,并且0.5%的品质改良因子处理组的菌丝体 SOD、POD、CAT、APX活性在培养的3~5d均高于对照组(P<0.05),说明添加0.5%品质改良因子培养的平菇不仅能够提高生物产量还能够显著提高菌丝体的抗氧化能力。
This study aimed to investigate the effect of different concentrations of stalk-derived quality modification factor (QMF) solution on the biomass of Pleurotus ostreatus mycelia over a 7-day culture period and the effects of 0.5% QMF solution on dynamic changes in DPPH and hydroxyl radical scavenging capacity, total antioxidant activity (T-AOC) and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX). The results showed that the 0.5% QMF treatment led to an increase in the mycelial dry weight of Pleurotus ostreatus by 19.31% on the 7th day compared with the control group. Additionally, despite no marked change in the DPPH radical scavenging activity, there was an 8%—11% increase in the hydroxyl radical scavenging activity after cultivation for 5, 6, 7 days, and an increase in the total antioxidant capacity up to 32% on the fifth day was also found during the first 5 days of cultivation compared with the control group. Moreover, the activities of SOD, POD, CAT and APX after cultivation for 3, 4, 5 days showed increased levels compared with the control group (P 〈 0.05). These observations show that treatment with 0.5% QMF solution can increase the mycelial biomass while apparently enhancing the antioxidant activity of Pleurotus ostreatus mycelia.