中文摘要：

目的构建多发性骨髓瘤（MM）黏蛋白MUCl-2VNTR基因的真核表达载体，为研制MM基因疫苗奠定基础。方法以MUCl-2VNTR的编码基因作为目的基因，在其前端加上COZAK序列后，两端分别加上HindⅢ和XbaI酶酶切位点，将人工合成的全基因定向克隆入pcDNA3．1／myc-hisB中，并通过酶切及测序进行鉴定。结果合成的MUCl-2VNTR基因全长约140bp，所构建的pcDNA3．11MUCl-2VNTR／myc-hisB重组体经双酶切及测序鉴定后，与预期片断大小相符，并含有完整的阅读框架和目的基因，证明构建成功。结论成功地构建了MM真核表达载体pcDNA3．1／MUCl-2VNTR／myc-hisB，为MUCl黏蛋白的功能研究和MM基因疫苗的研制奠定了相应的实验基础。

英文摘要：

Objective To construct multiple myeloma mucin MUC1-2VNTR gene eukaryotic expressing vector, which provided the basic material for further study of multiple myeloma DNA vaccine. Methods MUCl-2VNTR coding gene as larger gene, and a KOZAK sequence was inserted before it. Hind Ⅲ and Xba I restriction enzyme site were inserted on both ends. Then the whole sequence was synthesized and cloned into pcDNA3.1/myc-his B vector, and the recombinant vector was identified by restriction enzyme digestion and DNA sequencing. Results Synthesized MUCl-2VNTR gene was 140 bp. Restriction enzyme digestion and DNA sequencing confirmed pcDNA3.1/MUCl-2VNTR/myc-his B including the whole exact translation frame region and MUCI-2VNTR gene. Conclusion The pcDNA3.1/MUCl-2VNTR/myc-his B has been successfully constructed, which provides the basic material for further studies of MUCl mucin function and muhiple myloma DNA vaccine.