中文摘要：

目的观察黏蛋白1两串联重复区（MUC1-2VNTR）基因疫苗诱导BALB／c小鼠体液及细胞免疫应答的效果。方法将含MUC12VNTR的重组质粒peDNA3．1-2VNTR／myc-hisB免疫小鼠，免疫后采用ELISA动态检测血清中抗VNTR特异性抗体的水平，乳酸脱氢酶法检测细胞毒性T细胞（CTL）反应活性，MTS法检测脾淋巴细胞增殖活性。结果重组质粒免疫组小鼠血清中检出了MUC1-2VNTR抗原特异性抗体。用重组质粒免疫BALB／c小鼠后，在效靶比为80：1时可显著地诱导特异性CTL应答。在MUC1-2VNTR抗原肽的刺激下，免疫小鼠脾淋巴细胞得到增殖；与空质粒对照组比较，差异有统计学意义（P〈0．01）。结论MUC1-2VNTR基因疫苗可诱导小鼠产生特异性细胞及体液免疫应答，对多发性骨髓瘤的疫苗治疗有重要意义。

英文摘要：

Objective To observe the humoral and cellular immune responses induced by MUC1-2VNTR DNA vaccine in multiple myeloma（MM） mice. Methods Female BALB/c mice were immunized with pcDNA-2VNTR/myc-hisB. The levels of serum antibody specificity to MM cells were detected by using ELISA, cytolytic T lymphocyte（CTL） activity was detected by the method of LDH, spleen lymphocyte proliferation activity was detected by MTS/PMS method. Results Specific antibody against MUC1-2VNTR antigen was detected in serum of mice immunized with recombinant plasmid pcD- NA3.1-2VNTR/myc-hisB. When the ratio of effector cell to target cell reached 80： 1, specific CTL response was detected significantly in immunized mice. Compared with those immunized with empty vector, the spleen lymphocythes, which immunized with recombinant plasmid PeDNA3.1-2VNTR/myc-hisB, proliferated significandy（ P 〈 0.01 ）. Conclusions MUC1-2VNTR DNA immunization could elicit both humoral and cellular tumor specific immune response, which could be a possible clinical trial for the treatment vaccine of MM.