中文摘要：

目的探讨有效的分离与纯化大鼠骨髓间充质干细胞（BMSCs）方案，并研究BMSCs基本生物学特性。方法取SPF级4周龄健康SD大鼠1只，采用改良全骨髓贴壁法分离提取BMSCs，用倒置相差显微镜观察细胞形态变化及其生长特性，利用流式细胞仪检测P3代细胞表面标志物，细胞计数试剂盒（CCK）-8法绘制P3代细胞生长曲线。结果体外分离的原代细胞可见典型的贴壁生长，形态以梭形和多角形为主，呈旋涡状细胞集落，约8 d可达80%以上融合。传代纯化后细胞形态均一，具有较强的增殖能力。生长曲线提示BMSCs呈S形趋势。P3代 BMSCs代表面标志物分化群（CD）29、CD90阳性表达，而CD11b、CD45阴性表达。结论改良全骨髓贴壁法可获得高纯度、增殖活跃、生物学特性稳定的BMSCs，BMSCs的生长曲线呈S形趋势。

英文摘要：

ObjectiveTo explore an effective method for the isolation and purification of rats bone marrow mesenchymal stem cells （BMSCs） and to study the basic biological characteristics of BMSCs. MethodsOne healthy Sprague-Dawley （SD） rat of specific pathogen free （SPF） grade aged 4 weeks was selected, and the BMSCs were isolated using the modiifed whole bone marrow adherent method. The morphologic variation and proliferation features of BMSCs were observed under inverted phase contrast microscope, and the surface markers of BMSCs of P3 generation were detected by flow cytometry. Cell growth curves were drawn using the cell counting kit （CCK）-8 assay.ResultsTypical adherent growth was observed in the primary BMSCs with the forms of fusiform or polygon and appearance of circinate cell colony. The cell colonies fused with each other up to 80% in about 8 d. The puriifed passage cells still kept a homogeneous morphology and a high potential of proliferation. The growth curve of BMSCs indicated an S shape. The surface markers of BMSCs of P3 generation cluster of differentiation （CD） 29 and CD90 were observed positive, but CD11b and CD45 negative. ConclusionsBMSCs with high-purity, active proliferation and stable biological characteristics can be cultured using modiifed whole bone marrow adherent method. The growth curve of BMSCs presents in an S shape.