中文摘要：

NF-κB是具有多向性调节作用的蛋白质分子,NF-κB信号通路通过调控免疫相关基因的表达在鱼类先天性免疫中发挥重要作用,对该信号通路中2个关键信号分子NF-κB和IκB基因结构和表达模式研究非常重要。本文中,我们克隆了草鱼的NF-κB2和IκBα2个基因。氨基酸序列分析显示,NF-κB2含有1个RHD结构域、1个IPT结构域、6个ANKs结构域和1个Death结构域;IκB含有7个ANKs结构域。Real-time PCR分析发现,健康鱼体内NF-κB2在肝脏组织中表达量最高,IκBα在肾中表达量最高。GCRV病毒感染后,NF-κB2在肠中出现了显著的上调表达;IκBα在肝脏中产生了显著的下调表达,在肠中出现了小幅度的上调表达。说明NF-κB2和IκBα在病毒感染后的肝脏和肠中产生了主要的免疫应答,具体应答机制还有待进一步的研究。本研究结果为NF-κB2和IκBα在硬骨鱼类的抗病毒功能研究提供了重要线索。

英文摘要：

NF-κB is a protein molecule with multiple regulatory effects.NF-κB signaling pathway plays an important role in innate immunity of fish by regulating the expression of immune related genes.It is very important to study the structure and expression pattern of two key signaling molecules,NF-κB and IκB,in this signaling pathway.In this paper,we cloned the NF-κB2and IκBαgene of grass carp.Amino acid sequence analysis showed that NF-κB2contains 1RHD domain,1IPT domain,6ANKs domains and 1Death domain;IκBαcontains 7ANKs domains.Real-time PCR analysis showed that the expression of NF-κB2in liver tissue was highest,and the expression of IκBαin kidney was highest in healthy fish.After GCRV infection,the expression of NF-κB2in the intestine was significantly up-regulated;the expression of IκBαin the liver was significantly down regulated,and there was a small amplitude up-regulation in the intestine.These results suggest that NF-κB2and IκBαproduce a major immune response in the liver and intestine after virus infection,and the specific mechanism needs further study.The results of this study provide important clues for the antiviral function of NF-κB2and IκBαin teleost fish.