中文摘要：

为研究白细胞表面分化抗原81（CD81）的功能,对草鱼CD81进行了克隆,CD81全长共1376 bp,其中5′非翻译区87 bp,3′非翻译区581 bp,开放阅读框为708 bp,包括8个外显子,7个内含子,编码235个氨基酸。实验采用实时荧光定量PCR的方法检测了CD81在健康草鱼不同组织中的表达情况及草鱼出血病病毒（GCRV）攻毒前后的表达变化情况。结果显示草鱼CD81在所有被检测组织中均有表达,在头肾中表达量最高。在GCRV攻毒前后草鱼鳃、脾、肝、肠及头肾5个组织中的CD81表达量均有明显变化。同时,采用绿色荧光蛋白（GFP）来示踪CD81的亚细胞表达部位,激光共聚焦显微镜显示,同人类一样,草鱼CD81定位于细胞膜上。

英文摘要：

Cluster of Differentiation 81（CD81） is a transmembrane protein also known as tetraspanins that plays an important role in cell growth, differentiation, signal conduction and adhesion. In this study, we cloned the CD81 gene of grass carp. The length of the c DNA was 1376 bp containing an 87 bp 5′ untranslated region（UTR）, a 581 bp 3′ untranslated region（UTR）, and a 708 bp open reading flame（ORF）. The ORF had 8 exons and 7 introns, and encoded 235 amino acid residues. We applied real-time RT-PCR analysis to determine the pattern of CD81 gene transcription in different tissues of grass carp, and to measure the change in the transcript level after GCRV infection. The results showed that CD81 was expressed in all the tested tissues and was highest in head kidney. The transcript level was altered in gill, spleen, liver, intestines and head kidney after GCRV infection. Moreover, we used green fluorescent protein（GFP） to trace the subcellular expression of CD81, and the laser confocal electron microscopy revealed that grass carp CD81 was located on the cell membrane, which was the same in human.