中文摘要：

为研究贮脂类冬眠动物育肥过程和冬眠期糖代谢的机制,使用第二代转录组测序（RNA-Seq）技术,检测了达乌尔黄鼠起始育肥期、快速育肥期、育肥完成期和冬眠期4个阶段血糖含量和白色脂肪组织中与糖代谢途径相关基因的表达情况。结果显示,起始育肥期、快速育肥期、育肥完成期的血糖浓度无组间差异,但均高于冬眠期。与起始育肥期相比,快速育肥期的果糖-1,6-二磷酸酶基因表达下调了8.3倍,己糖激酶、醛缩酶和烯醇化酶等基因表达上调;育肥完成期与快速育肥期相比,果糖-1,6-二磷酸酶基因表达上调了9.6倍,醛缩酶、磷酸烯醇式丙酮酸羧化酶和柠檬酸合酶等基因表达下调1.2-2倍;冬眠期己糖激酶、丙酮酸脱氢酶E1、醛缩酶、柠檬酸合酶和6-磷酸脱氢酶等基因的表达均明显下调。结果表明,己糖激酶等基因表达上调可能与达乌尔黄鼠快速育肥期的糖代谢增强直接相关;醛缩酶和柠檬酸合酶等基因表达下调可能是动物在育肥完成期发生糖代谢降低的原因;入眠后,己糖激酶、丙酮酸脱氢酶等基因的低表达可能是调控糖代谢降至极低的主要机制。达乌尔黄鼠在冬眠前的活跃期既已启动糖代谢通路在分子水平上的主动调控。

英文摘要：

In order to study the mechanism of glycometabolism in the fattening progress and hibernation in fat-storing hibernators,we used next generation transcriptome sequencing technology（RNA-Seq） to detect differentially expressed genes associated with glycometabolism in the white adipose tissue of the Daurian ground squirrel（Spermophilus dauricus） and measured blood glucose at four physiological stages： Initial-Fattening,Rapid-Fattening,Finished-Fattening and Hibernation. Results show that there were no differences in blood glucose among stages of Initial-Fattening,Rapid-Fattening and Finished-Fattening but all were significantly higher than during Hibernation; Gene expression of fructose-1,6-bisphosphatase（FBPase） was down-regulated 8. 3-fold and a sampling of genes that were up-regulated at Rapid-Fattening compared with Initial-Fattening,included hexokinase（HK）,aldolase,enolase and so on; FBPase gene expression was upregulated 9. 6-fold and some other genes such as phosphoenolpyruvate carboxykinase,aldolase,citrate synthase at were down-regulated 1. 2-fold to 2-fold Finished-Fattening compared with Rapid-Fattening; expression levels of genes was significantly reduced in Hibernation,including hexokinase,aldolase,isocitrate dehydrogenase,6-phosphogluconate and so on.It suggests that the increase in expression of hexokinase and other enzymes could be directly linked to the enhancement of glycometabolism at Rapid-Fattening in the Daurian ground squirrel; the down-regulation of aldolase and citrate synthasegenes could be the reason for weakened glycometabolism at Finished-Fattening; the low expression of hexokinase,pyruvate dehydrogenase and other genes may be part of a general mechanism to coordinate glycometabolism reduction to an extreme level in hibernation. The intial regulation for glycometabolism pathways at the molecule level had been started in the active season before hibernation in the Daurian ground squirrel.