中文摘要：

目的：构建含锌指蛋白ZBTB20基因的重组腺病毒载体，以研究锌指蛋白ZBTB20的生物学功能。方法：将带有FLAG标签的ZBTB20的cDNA片段（ZBTB20-FLAG）克隆至pAdTrack-CMV载体，将该载体与pAdEasy质粒进行细菌内同源重组从而获得重组腺病毒载体pAd-ZBTB20，之后在293细胞中进行包装以及扩增，并对病毒滴度进行检测；采用肝脏组织特异性ZBTB20基因小鼠的原代肝细胞和肝脏组织模型，分别于离体和在体水平鉴定所制备的重组腺病毒Ad-ZBTB20介导的ZBTB20 蛋白的表达情况；并采用报告基因技术检测过表达的ZBTB20蛋白的功能活性。结果：成功制备了锌指蛋白ZBTB20重组腺病毒，该重组腺病毒感染原代肝细胞和小鼠肝脏组织能过表达ZBTB20蛋白，并且此ZBTB20蛋白能抑制其下游靶基因甲胎蛋白的转录。结论：所构建的ZBTB20重组腺病毒可以介导ZBTB20的过表达并具备转录调节活性，为今后研究ZBTB20的相关生物学功能奠定了基础。

英文摘要：

AIM： To construct the reeomlbinant adenovirus vector containing zinc finger protein ZBTB20 gene for the further study of the hiological function of ZBTB20. METHODS： The ZBTB20 cDNA fragment with FLAG tag was cloned into pAdTrack-CMV vector. The linearized vector was introduced into pAdEasy-1/BJ5183 bacterial cells for generaring homologous recombinant adenovirus plasmids, and was further transfeeted into HEK-293 packaging cells for the produetion of Ad-ZBTB20. The primary hepatocyles were used in the experimenh The expression anti activity of ZBTB20 prorein were determined hy Western blotting anti reporter gene assay alter Ad-ZBTB20 infection. The expression of ZBTB20 protein was also examined in the Liver tissue of liver-specific ZBTB20 knockout mice injected with Ad-ZBTB20 via the tail vein. RESULTS： Recombinant adenovirus Ad-ZBTB20 with high liter was successfully generated. Over-expression of ZBTB20 mediated by the recombinant was detected hoth in vitro and in vivo, with lhe aclivity of inhihiting the transcription of its target gene alpha-fetoprolein. CONCLUSION： Recombinant adenovirus Ad-ZBTB20 over-expresses ZBTB20 protein with the transcription regulation acctivity, which is a useful tool lot investigating the biological role of ZBTB20.