中文摘要：

利用表面增强拉曼光谱（SERS）技术研究了金属硫蛋白（MT）与脱金属硫蛋白（apo-MT）在粗糙金表面的吸附行为。结果表明：MT通过氨基与金作用;apo-MT与金的作用位点受制备方法影响：在溶液中预制的apo-MT通过半胱氨酸残基的巯基与金结合,在金表面制备的apo-MT通过氨基和巯基与金结合。SERS更适合于研究MT的结构特征。

英文摘要：

The adsorption of metallothionein and apo- metallothionein on roughened Au surfaces was studied by SERS technique. The results indicate that MT is adsorbed via - NH2 group on roughened gold surfaces. The adsorption site of apo - MT on Au is determined by the preparation method of apo - MT. The apo- MT prepared in solution is adsorbed via the - SH group on Au whereas that prepared on the Au surface is adsorbed via - SH and - NH2.