中文摘要：

为了执行机制，在解决方案和自我装配的单层的接口上万古霉素和 D-Ala-D-Ala-containing 肽的特殊协会学习，在万古霉素和 pentapeptide (Lys-Lys-Gly-D-Ala-D-Ala ) 之间的绑定被流动注射表面电浆子回声(FI-SPR ) 和流动注射石英水晶微量天秤(FI-QCM ) 调查。便于一个协议的形成有一致表面取向的万古霉素被吸附物质层，万古霉素分子被依附到 preformed alkanethiol 上自我装配的单层。由在装配薄片上为在 Lys-Lys-Gly-D-Ala-D-Ala 和万古霉素之间的绑定优化条件， Lys-Lys-Gly-D-Ala-D-Ala 的检测限制极大地被改进(到达 0.5 晡 ? 湵敤 ? 潴獲潩吗？

英文摘要：

To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer, the binding between vancomycin and pentapeptide （Lys-Lys-Gly-D-Ala-D-Ala） was investigated by flow injection surface plasmon resonance （FI-SPR） and flow injection quartz crystal microbalance （FI-QCM）. To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation, vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer. By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip, the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved （reaching 0.5 ×10^- 6 mol/L or 7.5 × 10^-12 mol）. The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained （KAds=5.0×10^4 L/tool）.