中文摘要：

目的探讨早幼粒细胞白血病蛋白（PML）和P53在食管鳞状细胞癌（ESCC）及癌前病变发生发展中的作用。方法应用组织芯片技术和免疫组织化学方法检测PML和P53在241例ESCC及癌前病变中的差异表达，分析蛋白不同表达模式与临床病理参数的相关性。结果在ESCC及癌前病变中，PML和P53呈阳性或强阳性表达，而在正常食管鳞状黏膜上皮中，呈阴性或仅在副基底层细胞有少量阳性表达。PML表达水平与淋巴结转移（X2=15．226，P〈0．001）、浸润深度（X2=29．461，P〈0．001）及临床分期（x2=26．956，P〈0．001）等有关。结论PML和P53在ESCC及癌前病变的发生发展中有重要作用。PML可作为一新的生物标记物，在ESCC及癌前病变的发生和演进中具有预警作用。

英文摘要：

Objective To assess the role of promyelocytic leukemia protein（PML） and P53 in the progressionof esophageal squarrous cell carcinoma （ESCC）and its precursor lesions. Methods Different expression patterns of PML and P53 of 241 cases of ESCC combined with adjacent precursors were analyzed by tissue array and immunohistochemistry and correlated with clinicopathological parameters. Results In ESCC and its precursor lesions,PML and P53 displayed positive or strong positive, while in normal esophageal epithelia, these proteins showednegative or stained positive only in parabasal cell layer. The expression level of PML was correlated with the depth of invasion of esophageal carcinomas （X2 =29. 461 ,P 〈0. 001 ）, lymph metastasis status （X2 = 15.226,P 〈 0.001 ）and pTNMs（x2 = 26.956,P 〈 0. 001 ）. Conclusion The abnormal expression levels of PML and P53 may play important roles in the carcinogenesis and evolution of ESCC and its precursors. The result suggests that PML, as a novel molecular biomarker, may be used to predict the development and progression of ESCC and its precursors.