中文摘要：

目的 探讨结直肠癌肝转移病灶钆塞酸二钠增强MRI纵向弛豫时间与肝细胞膜表面有机阴离子转运系统（OATP）、多耐药蛋白载体（MRP）含量的相关性.方法 前瞻性收集经肠镜活检病理证实为结直肠癌,CT增强检查发现肝转移灶,拟行肝转移瘤灶切除术的15例患者.患者病灶均为单发,于术前行上腹部平扫、DWI和钆塞酸二钠动态增强检查,在钆塞酸二钠注射前及注射后20 min（肝胆特异期）获得纵向弛豫时间图.测量计算肝转移灶和周围正常肝实质的钆塞酸二钠增强前、后纵向弛豫时间下降率（ΔT）.肝转移瘤灶切除术后获取肝转移灶和周围正常肝实质标本行蛋白质印记法检测,记录上述部位的OATP1、OATP8、MRP2、MRP3值.肝转移灶和周围正常肝实质间的OATP1、OATP8、MRP2、MRP3、AT采用t检验进行比较,患者OATP1、OATP8、MRP2、MRP3含量和ΔT值的相关性采用Spearman秩相关性分析.结果 病灶平扫T1WI呈相对低信号,T2WI呈相对稍高信号,DWI呈相对高信号,动态增强扫描动脉期病灶呈周边中度强化,肝胆特异期呈相对低信号.肝转移灶的OATP1、OATP8、MRP2、MRP2、ΔT值分别为（0.21 ±0.09）、（0.34±0.21）、（0.41±0.22）、（0.26±0.14）ng/ml和（33±9）％,周围正常肝实质上述值分别为（0.46±0.24）、（0.67±0.35）、（0.22±0.13）、（0.13±0.06）ng/ml和（68±9）％,差异均有统计学意义（t值分别为36.37、58.89、24.56、22.52和57.16,P均＜0.05）.患者的OATP1、OATP8、MRP2、MRP3值分别为（0.67±0.31）、（1.0±0.37）、（0.63±0.23）和（0.40±0.13） ng/ml,与ΔT（51±9）％均具有相关性（r值分别为0.491、0.432、-0.414和-0.371,P值分别为0.006、0.017、0.023和0.040）.结论 结直肠癌肝转移患者的OATP及MRP与钆塞酸二钠增强MRI纵向弛豫时间具有相关性.

英文摘要：

Objective To evaluate the relationship between the T1 relaxation times using gadoxetic acid-enhanced MRI with the organic anion transporter （OATP） and multi-drug resistance protein （MRP） values on colorectal liver metastasis and around normal liver parenchyma.Methods The perspective study included in fifteen patients with liver metastasis from colorectal cancer,who were confirmed colorectal cancer by colonoscopy,liver metastasis by CT scanning and would be underwent liver metastasis surgery.The lesion on the 15 patients was all single.The patients were performed plain scanning,diffusion weighted imaging and dynamic enhancement MRI examination using gadoxetic acid.Besides,for T1 mapping,a dual flip angle three-dimensional gradient echo sequence was performed before and at 20 minutes after gadoxetic acid administration.The reduction rate of T1 relaxation time （ΔT） （before and after gadoxetic-acid administration） of colorectal liver metastasis and around normal liver parenchyma was calculated.The specimens of hepatic metastases and around normal liver parenchyma were obtained during the colorectal liver metastasis operation.After that,they were analyzed by Western-blot and the values on OATP1,OATP8 and MRP2,MRP3 were recorded.The values on OATP1,OATP8 and MRP2,MRP3 and ΔT on hepatic metastases and around normal liver parenchyma were compared with t analysis.The correlation between the sum of the values （hepatic metastases adding around normal liver parenchyma） on OATPI,OATPS,MRP2,MRP3 with T1 relaxation times were calculated by Spearman.Results The scanning images on colorectal liver metastasis using gadoxetic acid MRI examination were as follows：relatively low signal intensity on T1WI; relatively higher signal intensity on T2WI; showing surrounding moderate enhancement on arterial phase and low signal intensity on portal venous phase,delay phase and hepatobililary phase.The values on OATP1,OATP8,MRP2,MRP3 and AT on the hepatic metastases were（0.21±0.09）,（0.34±0.21）,（0.41±0.22）