中文摘要：

目的：应用IDT公司的Antisense design software设计胶质细胞谷氨酸转运体-1a（GLT-1a）的反义寡核苷酸（AS-ODNs）,并对其进行效果评价。方法：首先根据GLT-1a mRNA羧基末端特异序列,设计GLT-1a AS-ODNs;在此基础上,应用Western blot方法对设计的5条GLT-1a AS-ODNs抑制大鼠海马CA1区GLT-1a蛋白表达的效果进行评价。结果：序列为5’-GGTTCTTCCTCAACACTGCA-3’的GLT-1a AS-ODNs可特异性地抑制大鼠海马CA1区GLT-1a蛋白表达,而对GLT-1b的表达无影响。该AS-ODNs对sham和头孢曲松钠（Cef）诱导的大鼠GLT-1a的表达上调的抑制效果类似,均〉60%;对脑缺血预处理（CIP）诱导的大鼠海马CA1区GLT-1a蛋白表达的上调亦具有明显的抑制作用,抑制效果〉60%。结论：从设计的5条GLT-1a AS-ODNs中获得了可特异性抑制GLT-la表达的反义寡核苷酸序列。

英文摘要：

Objective： The present study was undertaken to design antisense oligodeoxynucleotides （AS-ODNs） of glial glutamate trans- porter-la （GLT-la） and to evaluate the effectiveness of the designed AS-ODNs on the expression of GLT-la. Methods： Five sequences of GLT-la AS-ODNs were designed according to the C terminus specific sequences of GLT-la mRNA using antisense design software of IDT Com- pany. Western blot analysis was used to evaluate the inhibition effects of the five GLT-la AS-ODNs on the expression of GLT-la. Results： The sequence of GLT-la AS-ODNs with sequence of 5＇ -GGTFCTI＇CCTCAACACTGCA-3＇ could specifically inhibit the expression of GLT-la in the hippocampal CA1 subfield of rats, while it had no effect on the expression of GLT-lb. This sequence showed similar inhibition on the expres- sion of GLT-la in sham and ceftriaxone （Cef） -treated rats. It could also significantly inhibit the cerebral ischemic preconditioning （CIP） -in- duced up-regulation in the expression of GLT-la. The magnitude of the inhibition in sham, Cef- or CIP- treated rats was similar by more than 60%. Conclusion： From the designed five sequences of GLT-la AS-ODNs, we obtained an effective sequence which can specifically inhibit the expression of GLT-1a.