中文摘要：

目的观察p38 MAPK反义寡聚脱氧核苷酸（ASODNs）是否影响大鼠脑缺血预处理（CIP）诱导脑缺血耐受过程中GLT-1蛋白的表达。方法 80只健康Wistar大鼠,♂,永久凝闭椎动脉,随机分为6组：（1）假手术（sham）组（n=10）;（2）CIP组（n=10）;（3）脑缺血损伤（Ⅱ）组（n=10）;（4）CIP＋Ⅱ组（n=10）;（5）p38 MAPK AS-ODNs＋CIP＋Ⅱ组（n=30）;（6）p38 MAPK S-ODNs＋CIP＋Ⅱ组（n=10）。（5）组中根据p38 MAPK AS-ODNs所用剂量不同分为5 nmol、10 nmol及15 nmol亚组（n=10）;p38 MAPK S-ODNs的剂量为15nmol。所有动物均在sham手术或末次脑缺血/再灌注后6 h和2 d取材,Western blot和（或）免疫组织化学法检测p-p38MAPK及GLT-1蛋白的表达。结果 CIP引起p-p38 MAPK适度升高和GLT-1明显升高,并抑制Ⅱ引起的p-p38 MAPK过度升高及GLT-1表达下调;注射p38 MAPK AS-ODNs抑制CIP诱导脑缺血耐受过程中p-p38 MAPK和GLT-1的表达上调,呈剂量依赖性。结论 p38 MAPK AS-ODNs阻断CIP诱导脑缺血耐受过程中GLT-1表达上调。

英文摘要：

Aim To investigate the effect of p38 MAPK AS-ODNs on the expression of GLT-1 during the induction of brain ischemic tolerance induced by cerebral ischemic preconditioning（ CIP）. Methods Eighty healthy male Wistar rats with permanent occlusion of the bilateral vertebral arteries were randomly divided into 6 groups：（1）Sham group（ n = 10）;（2）CIP group（ n = 10）;（3）ischemic insult（ Ⅱ） group（ n = 10）;（4）CIP ＋ Ⅱ group（ n = 10）;（5）p38 MAPK AS-ODNs＋ CIP ＋ Ⅱ group（ n = 30）;（6）p38 MAPK S-ODNs ＋CIP ＋ Ⅱ group（ n = 10）. Group（5） was divided into 5nmol,10 nmol and 15 nmol subgroups according to the dose of p38 MAPK AS-ODNs（ n = 10）. The dose of p38 MAPK S-ODNs was 15 nmol. All the rats were sacrificed 6 h and 2 d after the sham operation or the last time of global brain ischemia reperfusion. Western blot and immunohistochemistry analysis were used for detecting the expression of p-p38 MAPK and GLT-1protein. Results CIP moderately up-regulated the expression of p-p38 MAPK and significantly up-regulated the expression of GLT-1 protein,inhibited the excessively up-regulation of p-p38 MAPK and the down-regulation of GLT-1 induced by ischemic insult. p38 MAPK AS-ODNs significantly inhibited the up-regulation of p-p38 MAPK and GLT-1 protein in a dose-dependent manner during the induction of brain ischemic tolerance by CIP. Conclusion p38 MAPK AS-ODNs inhibit the up-regulation of GLT-1 during the induction of brain ischemic tolerance induced by CIP.