中文摘要：

本文对268株结核分枝杆菌临床分离株（包括213株利福平耐药株、55株利福平敏感株）rpoB基因RRDR区进行测序并比较分析.结果表明,在213株利福平耐药株中有200株（93.90%）在rpoB基因RRDR区发生了突变,在55株利福平敏感株中RRDR均未发生突变.突变频率最高的类型依次为：531位点（55.87%）,526位点（16.43%）,516位点（10.33%）和511位点（8.92%）,以上四个位点的突变菌株数占所有利福平耐药株的91.55%.四川地区利福平耐药株中511位点突变频率高于全国其他地区.

英文摘要：

Sequencing of the rpoB gene was performed for 268 randomly selected isolates,including 213 rifampin-resistant and 55 rifampin-susceptible isolates.Mutations in the RRDR of rpoB gene were detected in 200（93.90%） of 213 rifampin-resistant isolates and none of the 55 rifampin-susceptible isolates.The most common mutations were in codon 531（55.87%）,526（16.43%）,516（10.33%） and 511（8.92%）,together accounting for 91.55% of rifampin-resistant isolates.It is demonstrated that mutation frequency of codon 511 in Sichuan was higher than in other provinces of China.