中文摘要：

目的建立叠鞘石斛中联苄类成分的定性、定量分析方法。方法运用薄层色谱法对叠鞘石斛中联苄类成分石斛酚（gigant01）和杓唇石斛素（moscatilin）进行定性分析，采用高效液相色谱法测定石斛酚的含量，以Shim—packCLC—ODS柱为固定相，流动相乙腈-0．2％甲酸（35-65），检测波长280nm，柱温25℃。结果石斛酚在0．196～12．54μg内峰面积与进样量呈良好的线性，r=0．9999；平均回收率100．6％；RSD3．0l％。结论通过10批不同来源的叠鞘石斛的鉴别和含量测定，证明方法灵敏，准确可靠，重复性好。在不同采收期的叠鞘石斛中，石斛酚的含量在春季4，5月份和秋季10，11月份相对高于其他月份。

英文摘要：

OBJECTIVE To establish qualitative and quantitative methods for the quality evaluation of Dendrobium aurantiacum var. denneanum. METHODS Two bibenzyls gigantol and moscatilin were identified by TLC. The separation of gigantol was obtained by RP-HPLC （ Shim-pack CLC-ODS） using elution systems of acetonitrile-0. 2% formic acid （65： 35） at 25 ℃. RESULTS Calibration curve showed good linear regression （r = 0. 999 9） within test ranges of 0. 196 - 12. 54 μg. Average recovery was 100. 6% with RSD of 3.01%. CONCLUSION The developed methods were proved to be sensitive, reliable and useful by analysis of ten different samples from D. aurantiacum vat. denneanum. The samples collected in April, May （Spring） and October, November （Autumn） accumulate much higher amount of gigantol than those of other months.