中文摘要：

排序的 nexins (SNX ) 为细胞内部的蛋白质排序，交通国王和信号 transduction 是 PX 领域包含蛋白质和必需品。SNX 的 PX 领域能把鼓舞的 phosphoinositides (PI ) 绑在各种各样的黄磷并且指向主人蛋白质到内涵体。最近，我们报导了那能在在哺乳动物的房间的 SNX10 的表示上导致巨大的液泡。在这研究，我们试图在为 vacuolation 活动批评的 SNX10 识别区域。我们发现 PX 领域和 CD1 区域为 vacuolation 是必要的。我们提供了 PX 领域能明确地绑在 PtdIns (3 ) P 和目标 SNX10 到内涵体的证据。在 PX 域(V15A ) 的 β1 区域的一个变化破坏了 PtdIns (3 ) P 有约束力的能力和 SNX10 的 endosomal 本地化。然而，改正细胞的本地化独自不是的潜水艇为 SNX10 到足够导致液泡。我们发现 CD1 区域，没为本地化被要求，为 SNX10 的 vacuolation 活动是不可缺少的。在摘要， PX 领域和 CD1 区域是必要的让 SNX10 导致液泡，但是他们在这个过程起不同作用。

英文摘要：

Sorting nexins （SNXs） are PX domain containing proteins and essential for intracellular protein sorting, trafficking and signal transduction. The PX domains of SNXs can bind to various phosphorelated phosphoinositides （PIs） and target the host proteins to endosomes. Recently, we have reported that overexpression of SNX10 in mammalian cells could induce giant vacuoles. In this study, we aimed to identify regions in SNX10 critical for the vacuolation activity. We found that both the PX domain and the CD1 region were essential for vacuolation. We provided evidence that the PX domain was able to specifically bind to Ptdlns（3）P and target SNX10 to endosomes. A mutation in the 131 region of the PX domain （V15A） disrupted the Ptdlns（3）P binding ability and the endosomal localization of SNX10. However, correct subcellular localization alone was not sufficient for SNX10 to induce vacuoles. We found that the CD1 region, which was not required for the localization, was indispensable for the vacuolation activity of SNX10. In summary, both the PX domain and the CD1 region are necessary for SNX10 to induce vacuoles but they play different roles in this process.