中文摘要：

本研究报道了以庐山野生蛇足石杉成年株新生枝茎为外植体,运用植物离体培养技术获得了产生石杉碱甲的叶状体。实验结果表明：适宜叶状体发生的培养基为6,7-V＋IAA 0.5 mg/L,诱导率达50%;叶状体增殖的最佳培养基是无激素的MS（其中蔗糖用量20 g/L）,叶状体继代培养55 d,其相对增殖率达1362.6%（即收获量是接种量的14.6倍）。通过薄层层析（TLC）和高效液相（HPLC）法检测叶状体提取物中的目标成分,表明本研究建立的叶状体具有累积与原植物相同的有效成分石杉碱甲的能力。

英文摘要：

In this research, in vitro culture method was developed for obtaining Huperzia serrata thallus which producets Huperzine A, an anti-Alzheimer＇ s disease （AD） drug candidate. The semi-woody branches of wild mature plant of hu- perzia serrata from Lushan were chosen to be the test material. The test results indicate that the suitable medium to in- duce thallus was 6,7-V ＋ IAA0.5 mg/L,and its differentiation rate was 50%. Also,MS minimal medium without any hormone was established as the optimal subculture medium. Given sugar amount of 20 g/L in this subculture medium, the relative increasing rate of thallus Was 1362.6% after 55 days, namely 14.6 folds biomass of inoculation. Through Thin-layer Chromatography and High Performance Liquid Chromatography detections,the production of active ingredients Huperzine-A by the obtained thallus has been confirmed.