中文摘要：

本文主要研究不同蛋白质饲料对瘤胃微生物蛋白产量及其氨基酸（AA）组成模式影响的规律。以4只瘘管山羊作为试验动物，分为A组（羽毛粉组）、B组（玉米蛋白粉组）、C组（豆粕组）和D组（鱼粉组）4个组，进行4×4拉丁方试验。结果表明：不同蛋白质饲料显著影响瘤胃微生物蛋白的量，以豆粕组最高，为13．9750mg／mL，羽毛粉组最低，为10．7650mg／mL（P〈0．01）。微生物蛋白中AA-N的含量也受蛋白质来源的影响，豆粕组AA—N比例最低，而鱼粉组最高。进一步回归分析表明AA—N的含量与细菌（原虫）蛋白有负（正）相关关系，回归方程为Y=15．469-1．098X1＋1．498X2（R^2=0．944）。研究还发现微生物蛋白的AA组成模式在不同蛋白质处理以及微生物区系间有差异。原虫蛋白的Val含量高于细菌，而细菌蛋白的Lys含量则高于原虫。细菌蛋白的Arg含量，原虫蛋白的Met、Leu和His含量在各组间差异显著。AA的变化与微生物类群的变化有一定的关联。因此，日粮蛋白质对瘤胃微生物蛋白产量和微生物AA组成都有一定的影响。

英文摘要：

The main objective was to study the variation in the amino acid （AA） profile of microbial fractions obtained after feeding 4 kinds of dietary protein feedstuffs to Xuhuai Goat. Four goats fitted with rumen cannula were used in a 4 × 4 Latin square design. And artificial diets were divided into 4 groups according to the nitrogen source： group A （feather meal group）, group B （corn gluten meal group）, group C （soybean meal group）, and group D （fish meal group）. Results showed that, yields of microbial crude protein （MCP） varied with dietary protein, and the lowest was in group A （10. 765 0 mg/mL）, while the highest was in group C （13. 975 0 mg/mL） （P〈0.01）. The microbial AA-N ratio was also alerted by dietary pro- tein. Group C recorded the lowest value, and the opposite was found in group D that recording the highest peak. Further multiple regression analysis showed that AA-N ratio （Y） had a negative/positive correlation with bacteria （X1）/protozoa （X2） group, and the regression equation was.. Y = 15. 469 - 10. 098X1 ＋ 1. 498X2 （R^2 = 0. 944）. It was also observed that, across all rations, significant differences were found between protozoa and bacteria in AA content for some but not all amino acids. Such as protozoal Val was significantly higher than that of bacterial; while bacterial Lys was significantly higher than that of protozoal. Additionally, within each microbial fraction, significant differences in the content of AA were found among rations. For bacteria, significant difference was detected in Arg only （P〈0.05）; for protozoa, Met, Leu and His showed significant differences （P〈0.05）. And the variations of AA were related to the changes of microbial population in some degree. It was concluded that yields, AA-N ratio, and AA profile of ruminal MCP were all affected by dietary protein.