中文摘要：

建立了同时检测人尿液中7种邻苯二甲酸酯代谢物的高效液相色谱-串联三重四极杆质谱法。尿液经酶水解后,采用萃取柱净化,以2%（v/v）甲酸甲醇溶液为洗脱剂,经苯基柱分离,以0.1%（v/v）乙酸水溶液和0.1%（v/v）乙酸乙腈溶液为流动相进行梯度洗脱,采用电喷雾离子源负离子模式和多反应监测模式采集信号,用同位素内标法进行定量分析。尿液中7种邻苯二甲酸酯代谢物在0.2-200.0μg/L范围内定量离子的相对峰面积比值与质量浓度均呈良好线性关系（r≥0.999 76）;检出限（LOD）为13.43-80.21 ng/L,定量限为44.77-267.37 ng/L;3个水平的加标回收率为88.8%-108.9%,日内和日间精密度均不大于17.05%。该方法可同时准确、灵敏、简便地测定人尿液中7种邻苯二甲酸酯代谢物的暴露水平。

英文摘要：

A method has been developed for the analysis of seven metabolites of phthalates in human urine by high performance liquid chromatography-tandem mass spectrometry（ HPLC-MS/MS）. The urine samples were hydrolyzed with glucuronidase followed by purification with solid-phase extraction（ SPE）cartridges. Both 0. 1% formic acid in water（ v/v）and 0. 1% for-mic acid in acetonitrile were used as the mobile phases in a gradient mode. The chromatograph-ic separation was achieved on a phenyl column. Mass detection was then conducted by electros-pray ionization in negative ion mode and multiple reaction monitoring mode. The components were quantified by stable isotope-labelled（ ^13 C-）phthalate monoester internal standards. The calibration curves of the seven phthalates metabolites showed good linear relationships in the range of 0. 2-200. 0 μg/L（ r﹥0. 999 76 ）. The recoveries at three levels were from 88. 8% to 108. 9% with relative standard deviations no more than 17. 05%. The limits of detection of the method were 13. 43-80. 2 ng/L. The limits of quantification were 44. 77-267. 37 ng/L. This method was successfully applied to the determination of metabolism of phthalates in human urine with efficiency,increased accuracy and high sensitivity.