中文摘要：

目的：考察提高CXCR7表达能否提高内皮祖细胞（EPCs）血管生成能力。方法：利用高表达CXCR7的腺病毒转染至人脐带血来源的EPCs,建立CXCR7high-EPCs工程化细胞。比较CXCR7high-EPCs与对照组EPCs在无血清条件下存活、黏附、经基质细胞衍生因子-1（SDF-1）诱导的跨内皮迁移、管样结构形成以及一氧化氮（NO）生成能力。结果：高表达CXCR7能显著增强EPCs在无血清条件下细胞存活能力、黏附功能、SDF-1诱导的跨内皮迁移、管样结构形成以及EPCs生成NO的能力,并且与SDF-1有协同作用。结论：提高CXCR7在人脐带血来源的EPCs中表达能够增强其血管生成能力,这为临床治疗血管性疾病提供新思路。

英文摘要：

Objective： To investigate whether increasing CXCR7 expression in endothelial progenitor cells （EPCs） can improve its angiogenic capability. Methods： CXCR7M~H-EPCs were constructed by transfecting with adenovirus carring human CXCR7 gene. The differences between CXCR7high-EPCs and wt-EPC in cell survival under serum deprivation condition, adhesion, SDF-1 induced trans-endothelial migration, tube formation and ni- tric oxide （NO） production were compared. Results： Compared with ctrl-EPCs, CXCR7high-EPCs has superiority in cell survival under serum-free condition, cell adhension to activated endothelial cells, trans-endothelial migra- tion, tube formation and NO production, which could be further enhanced by SDF-1. Conclusion： Increasing expression of CXCR7 can enhance the angiogenic capacity of EPCs. The performance of present research may provide a new idea for the treatment of vascular diseases.