中文摘要：

鉴定和获取了四种油料作物（油菜、大豆、花生和芝麻）中的细菌型PEPC基因，分析了所编码蛋白的保守结构域（BOXI-IV）和蛋白作用功能位点。基因包括甘蓝型油菜的Bna10093361、Bna1009749和Bna10093360，大豆的Clylmag10g70.1，Glyma01g22840．1和Glyma02g14500．1,芝麻的SINl018296和花生的AhPPC5。这8个基因通常含有19～21个内含子，内部插入一个约350～600bp的高度变异区，编码的蛋白在C端形成R／KNTG结构域，在N端缺乏磷酸化作用位点。在种子发育的不同时期，油菜中仅Bnal0093360表达，但其表达量不到油菜Bn-Actin表达量的0．1％；大豆中Glymal0g34970．1 表达量最高（接近大豆GlymaActin的2％），Glyma02g14500．1次之；花生AhPPC5表达量为花生AhActin的32％～175％，在种子不同发育时期表达量为早期〉中期〉晚期；芝麻SINl018296表达量为芝麻SINActin的3％-18％，在种子发育时期的表达趋势和花生AhPPC5相似。8个基因种子中的表达模式差异明显，说明细菌型PEPC基因可能存在着广泛的功能分化。

英文摘要：

Bacterial-type PEPC genes were cloned from four oil crops （rape, soybean, peanut and sesame）. Their sequences of conserved domains （ BOX I to BOX IV） and PEPC activity sites were characterized. The genes included BnalO093361, BnalO09749 and BnalO093360 from Brassica napus, GlymalOg34970. 1, GlymaOl g22840. 1 and GlymaO2g14500.1 from Glycine max, SIN1018296 from Sesamum indicum, AhPPC5 from Ara- chis hypogaea. The 8 genes were commonly composed of 19 to 21 introns, and contained one unique and highly di- vergent insertion of about 356 to 600bp. PEPC sequences had R/KNTG in C-terminal tetrapeptide, but lacked the phosphorylation motif at N terminus. Results from RT - qPCR showed that BnalO093360 expressed with less than 0.1% of rapeseed BnaActiu, GlymalOg34970. 1 and GlymaO2g14500. 1 expressed with less than 2% of soybean GlymaActin. AhPPC5 expressed 32% to 175% of peanut AhActin, and SIN1018296 expressed 3% to 18% of sesame SINActin. The expression trends of AhPPC5 and SIN1018296 were higher at early seed developing stage and then decreased. Results indicated that the bacterial-type PEPC gene functions were widely differentiated.