中文摘要：

为丰富芝麻EST-SSR引物，本研究从实验室构建的高油芝麻品系中芝14号的不同种子发育期cDNA文库中获得45569条表达序列标签（expressed sequence tags，EST），通过前处理得到总长17.428Mb的无冗余EST共32421条。利用MISA和SSR finder软件包工具对EST序列进行SSR位点查找，在这些序列中发现有1688条Unigene，共有1949个SSR。共226个序列含2个或2个以上SSR位点。这些SSR中出现频率最高的重复基序类型为三核苷酸（74％）。为开发芝麻EST-SSR引物，探讨EST-SSR用于芝麻品种遗传差异研究的可行性，用12份不同含油量品系材料评价其中34对与油脂代谢相关的基因SSR引物，全部成功扩增，共有3对引物获得多态性条带。结果表明，芝麻EST-SSR标记开发效率较高，对于芝麻品种的鉴定与遗传多样性分析具有很高的应用价值。

英文摘要：

Currently, the lack of EST sequence information has limited the discovery of sesame EST-SSR primer number. All the 45 569 EST sequences of high oil content sesame species Zhongzhil4 were obtained from a full-length developing seeds eDNA library and assembled 32 421 unigenes. The unigenes represented about 17.428 Mb of genic sequences. Using MISA and SSR finder software to screening SSRs, 1 949 non-redundant （NR） SSRs were identified in 1 688 uniESTs. A total of 226 sequences contained more than one SSR. The trinu-cleotide repeats are the dominant types with the frequency of 74%. All of the designed 34 SSR primer pairs related to oil synthesis genes could amplify characteristic SSR fragments against 12 different oil content species, and 3 primer pairs deal amplified polymorphic fragments. The EST-SSR markers of sesame are highly efficiency, and should be the itools for studies of genetic variation, genetic diversity, and development of genetic linkage maps of sesame.