中文摘要：

目的探讨胆固醇对血管平滑肌细胞增殖及迁移的作用。方法体外培养人血管平滑肌细胞株（VSMC）,以12.5 mg/L、25.0 mg/L、50.0 mg/L不同浓度胆固醇（CH）分别作用细胞24 h、48 h、72 h后,MTT法检测VSMC的增殖情况;流式细胞术检测细胞进入S期的数量;EDU试剂盒检测CH作用后细胞的DNA合成;划痕实验检测VSMC迁移情况;Real-time PCR检测细胞中miR145的表达水平。结果 12.5 mg/L、25.0 mg/L、50.0 mg/L CH作用VSMC后可增加细胞活力,促进细胞增殖,与对照组比较,进入S期的VSMC细胞数量明显增多,细胞迁移增加（P〈0.05）;与对照组比较,25.0 mg/L、50.0 mg/L CH可诱导VSMC DNA合成增加,并且明显降低miR-145的表达水平（P〈0.05）。结论 CH可诱导VSMC进入S期细胞增多,促进细胞增殖,并且增加细胞的DNA合成及细胞迁移,其机制可能与抑制miR-145的表达有关。

英文摘要：

Aim To study the effects of cholesterol （CH） on proliferation and migration of vascular smooth muscle cells （VSMC）.Methods VSCMs were treated with 12.5 mg/L,25.0 mg/L,50.0 mg/L CH.VSMC activity were analyzed by MTT kit,the percentage of DNA synthesis were detected by EdU kit,the percentage of S phase cell numbers were measured by flow cytometry and VSMC migrations were detected by scratch assay.The expression of miR145 was analyzed by real-time quantitative PCR.Results 12.5 mg/L,25.0 mg/L,50.0 mg/L CH can increase VSMC proliferation,migration and the percentage of S phase cell numbers （P〈0.05）.25.0 mg/L,50.0 mg/L CH promoted DNA synthesis percentage of VSMC,decreased the expression of miR-145 in VSMC （P〈0.05）.Conclusion Cholesterol increased the percentage of S phase VSMC number,cells proliferation,DNA synthesis and migration,and miR-145 may be involved in these processes.