中文摘要：

为建立杉木SRAP-PCR反应体系，利用L15（4^5）正交设计对影响杉木SRAP-PCR反应体系的Mg^2＋、dNTPs、引物、Taq酶和DNA浓度5种因素的4个水平进行优化实验，结合正交直观分析和方差分析，对影响反应较大的Mg舢、dNTPs和引物浓度进行单因素实验，最终确定杉木SRAP-PCR最佳的反应体系为：在20μL的PCR反应体系中，Mg^2＋’浓度为2．25mmol／L、dNTPs为0．15mmol／L、引物浓度为0．4Ixmol／L、Taq酶为1．5txmol／min、模板DNA为60ng，10xPCRBuffer2IxL，不足部分用双蒸水补充至20μL。PCR反应程序的两步最适退火温度第1步为35℃，第2步为53℃。利用上述反应体系进行杉木PCR扩增，能得到清晰、稳定的条带。

英文摘要：

In order to optimize SRAP-PCR reaction system for Chinese fir,the orthogonal design L16（4^5） was considered with different concentrations of Mg^2＋, dNTPs, primer, Taq DNA polymerase and DNA template in SRAP-PCR reaction system for Chinese fir. The results of PCR were evaluated by visual and variance analysis ,then the concentrations of Mg^2＋, dNTPs,primer were rectified with single-factor design. Finally, the results showed that the best reaction system was the combination in 20 μL reaction solution, containing of Mg^2＋ 2.25 mmol/L, dNTPs 0.15 mmol/L, primer 0.4 μmol/L, Taq DNA polymerasel.5 p.mol/min and DNA template 60 ng,2 μL10xPCR Buffer and ddH20 to 20 μL. The fimt optimal an- healing temperature was 35 ℃ ,and the second step was 53 ℃. The optimized reaction system of Chinese fir described a- bove can get clear and stable bands.