中文摘要：

目的优化生姜高效液相色谱指纹图谱,为其质量控制提供可靠依据。方法采用Aichrom Bond-AQ C18（4.6mm×250mm,5μm）色谱柱,以甲醇（A）和水（B）流动相,梯度洗脱（0~8min,25%A;8~20min,25%A→60%A;20~40min,60%A→70%A;40~70min,70%A→88%A;70~80min,88%A→90%A;80~85min,90%A）,检测波长280nm,流速1ml/min,柱温40℃。采用＂中药色谱指纹图谱相似度评价系统软件（2004A版）＂进行指纹图谱分析。结果分别优化色谱柱、检测波长、流动相和HPLC梯度洗脱条件,标定了15个共有指纹特征峰,指认了6-姜酚、8-姜酚、香兰素3个色谱峰,建立10个批次的生姜HPLC-DAD色谱指纹图谱,相似度均大于0.9。结论该方法经方法学考察符合指纹图谱技术要求,为有效地控制生姜的品质评价和质量控制提供参考依据。

英文摘要：

Objective To optimize the fingerprint of Zingiber officinale using HPLC-DAD technology for quality control. Methods The chromatographic fingerprints were obtained by the Aichrom Bond-AQ C18（ 4. 6mm × 250 mm,5μm） with the mobile phase of methanol（ A）-water（ B） for gradient elution（ 0-8min,25% A; 8 ~ 20 min,25% A→60% A; 20 ~ 40 min,60% A→70% A; 40 ~ 70 min,70% A→88% A; 70 ~ 80 min,88% A→90% A; 80 ~ 85 min,90% A）. The wavelength was set at 280 nm and the column temperature was maintained at 40℃. The flow rate was 1. 0 ml·min-1. The similarity assay of Zingiber officinale of different sources was carried out to evaluate their quality by traditional Chinese medicine fingerprint similarity evaluation system（ 2004 A edition）. Results Totally 15 common fingerprint peaks and 3 constituents including 6-Gingerol,8-Gingerol and Vanillin were identified after optimization of the chromatographic column,detection wavelength,mobile phase and HPLC chromatography gradient elution conditions. Moreover,the similarity of HPLC fingerprints of 10 different batches of Zingiber officinale was over 0. 9. Conclusion The present methodology meets the technical requirements of fingerprint and could be used for quality evaluation and control of the fresh fruits of Zingiber officinale.