中文摘要：

目的 研究在结肠癌细胞系中Hrs与凋亡及增殖的关系。方法 在结肠癌细胞系HCT-116和SW-480中下调Hrs蛋白表达,通过Incucyte、MTT法、软琼脂试验、FACS检测细胞凋亡与增殖情况。结果 下调Hrs表达后,结肠癌细胞系HCT-116和SW-480凋亡明显增加,增殖及细胞活性均显著受到抑制。结论 Hrs与结肠癌细胞凋亡密切相关。

英文摘要：

Objective To study the effects of Hrs on apoptosis and cell proliferation in colon cancer cell lines HCT- 116 and SW-480. Methods The expression of Hrs in HCT- 116 and SW-480 was knocking down by shRNA. Cell apoptosis was detected by FACS and cell proliferation was de- telmined by MTF, incucyte and soft agar. Results The apoptosis was significantly increased after Hrs knocking down in both HCT-116 and SW- 480, and the cell proliferation was inhibited. Conclusion Hrs is involved in the apoptosis and proliferation of colon cancer cells.