中文摘要：

目的 研究PERl蛋白对NIH3T3细胞增殖和迁移的影响。方法 将重组表达质粒pcDNA3．1／mPERl转染入NIH3T3细胞中．并以未转染的NIH3T3细胞为对照．利用MTT法检测细胞增殖的改变。利用RT-PCR技术和Westernblot检测节律蛋白mPERI对细胞迁移关键性蛋白基质金属蛋白酶2（MMP-2）表达的影响。结果 MITT法显示PERl表达上调可以抑制NIH3T3细胞增殖．RT—PCR技术和Westernblot检测显示在NIH3T3细胞中。当节律蛋白roPERl表达上调时，细胞迁移关键性蛋白MMP-2在RNA和蛋白水平的表达较对照组降低。结论 上调NIH3T3细胞内的PERl蛋白表达能抑制细胞增殖以及细胞迁移的关键性MMP-2在RNA和蛋白水平的表达。

英文摘要：

Objective To study the function of mPERI on cell growth and migration of NIH3T3 cells. Methods The peDNA3, l/roPER1 constructed plasmid was transferred into NIH3T3 cells by the Lipofectaml TM 2000 reagent and con- trolled by the NIH3T3 cells without peDNA3.1/mPERI, The cell growth of these two groups was detected by MTT and the expression of cell migration proteln-MMP-2 was analyzed by RT-PCR and Western blot. Results NIH3T3 cell proliferation was inhibited when mPER1 expression was up--regulated. The level of MMP-2 expression in NIH3T3 cells was decreased after transfection with the pcDNA3.1/mPERI plasmid. Conclusion The cell growth of NIHST3 and the expression of cell migration protein MMP-2 will be decreased when mPERI expression is up-regulated.