中文摘要：

目的 探讨多球壳菌素（ISP-Ⅰ）对体外激活的大鼠肾小球系膜细胞（GMC）的调控作用。方法 分别采用MTT法及流式细胞术检测IL-1、IL-6对GMC的激活作用及ISP-Ⅰ对激活后GMC增生的抑制作用。结果 MTT法结果显示10U／ml IL-1及1000U／ml IL，6刺激GMC24、48h后，反映细胞增生程度的吸光度（A）值较对照组增高（P〈0．05）；激活的GMC在25～500μg／ml ISP-Ⅰ作用24、48h后的A值较对照组低（P〈0．05）。流式细胞术检测的结果与MTT法一致，即GMC被激活12、24、36h时的细胞增生率高于对照组（P〈0．05）；激活的GMC在100μg／ml ISP-Ⅰ作用下，细胞增生率低于对照组（P〈0．05）。结论 ISP-Ⅰ对激活后的GMC有明显抑制作用。

英文摘要：

Objective To study the effects of myriocin （ISP-Ⅰ） on activated glomerular mesangial cells （GMCs） in rats. Methods MTT method and FCM were used to determine proliferation rate of GMCs stimulated by IL-1, IL-6 and the anti-pro- liferation rate of ISP-Ⅰ on activated GMCs. Results The absorbance （A） value, which represented the cell proliferation rate, of GMCs stimulated by IL-1 and IL-6 was higher than in control group. The A value of activated GMCs inhibited by ISP-Ⅰ was lower than in GMCs activated by IL-1, IL-6; FCM revealed the percentages of proliferative GMCs stimulated by IL-1, IL-6 were higher than those in the controls. FCM also indicated the percentages of proliferative cells in ISP-Ⅰ treated group were lower than in those stimulated by IL-1, IL-6. Conclusion ISP-Ⅰ can significantly inhibit the activated GMCs.