中文摘要：

目的对原核系统表达的重组刚地弓形虫Peroxiredoxin（rTgPrx）蛋白进行镍亲和层析纯化,免疫大鼠,制备抗血清。方法优化rTgPrx表达条件,获得大量的可溶性蛋白,经镍亲和层析法纯化后皮下注射免疫Wistar大鼠,间接ELISA法测定血清抗体效价。结果在表达菌液A600为0.5,IPTG浓度为0.2 mmol/L,37℃诱导10 h时,可溶性rTgPrx表达量较高;用镍亲和层析纯化获得纯的目的蛋白,免疫大鼠后诱导产生高滴度抗体血清,效价达1∶12 800以上。结论镍亲和层析法纯化的rTgPrx具有免疫原性,用该蛋白免疫大鼠可获得高效价的抗rTgPrx血清。

英文摘要：

Objective To purify recombinant peroxiredoxin of Toxoplasma gondii expressed in prokaryotic cells with Ni-NTA affinity chromatography and immune rats and prepare antisera.Methods Conditions for expression of rTgPrx were optimized.Large quantities of soluble rTgPrx were purified with Ni-NTA affinity chromatography.Rats were immunized with purified rTgPrx through subcutaneous injection.The antibody was detected by indirect ELISA.Results Soluble rTgPrx was induced by 0.2 mmol/L IPTG administered for 10 h at 37 ℃;with an A600 of 0.5,relatively large quantities of soluble rTgPrx were expressed.Rats immunized with the purified rTgPrx exhibited a high titer antibody.The titer of prepared antisera was 1∶12 800.Conclusion rTgPrx that was purified with Ni-NTA affinity chromatography was found to be immunogenic.High-titer antisera were obtained by immunizing rats with the purified rTgPrx.