中文摘要：

蛋白质糖基化是一种广泛存在的重要的蛋白质翻译后修饰,糖基化肽在总酶解肽中占的比例不超过5%,这使得糖肽的分离富集成为糖蛋白质组学研究发展的关键技术之一。在诸多糖蛋白质组富集技术中,化学方法是富集技术的主导,本文从化学反应的角度介绍糖基化蛋白质富集的技术进展。富集过程按照连接和释放分别讨论,在连接过程中,重点介绍硼酸化学法、肼化学法、胺化学法和肟点击法;在释放过程中,以N-糖蛋白的酶释放法和O-糖蛋白的β-消除法为主导,一并介绍了最新的氧化断裂释放的化学法。最后,讨论总体富集策略的发展现状。该文以糖蛋白富集的共价反应为核心,分析不同方法的优缺点以及各技术在糖蛋白质组学研究中的应用和贡献。

英文摘要：

Protein glycosylation is one of the most common and important post-translational modifications （PTMs） in mammalian cells. Glycopeptides usually exist at relatively low abundance （less than 5%） compared with non-glycosylated peptides. So,enrichment approaches are important for the analysis of glycoproteome. Approaches based on chemical reactions are the main trend,here we discuss them from chemical viewpoint. There are two main processes for enrichment of glycoproteins,glycoprotein conjugation and the release from solid phase. In con-jugation process,we introduce four types of chemical reactions ： one is boronic acid chemistry based on reaction between boronic acid and cisdiol groups on free glycans, and the other three are covalent reactions between aldehyde groups and functionalized groups, hydrazide for hydrazone ligation,alkylamine for reductive amination,aniline for nonreductive amination and aminooxy group for oxime click chemistry. In release process,N-glycosidase （PNGase） F for N-glycosylated proteins,β-elimination reaction for O -glycosylated proteins and a bleach method published recently are introduced,and the oxidative releasing of natural glycans is also introduced here. In addition,analysis strategies for N-/O -glycoproteins are discussed,including detection in glycoproteins and site-specific glycan occupancies. This review mainly focuses on the currently available chemical approaches based on covalent reactions for enrichment of gly- coproteins,as well as new materials derivatized with different functional groups. We compare the advantages and disadvantages of different methods and try to evaluate the contribution and future trend based on authors viewpoint.