中文摘要：

一个金 immunochromatographic 传感器(GICS ) 在蜂蜜样品为 26 硫安类药剂的快速的察觉被开发。传感器基于能认出所有 26 硫安类药剂的组特定的 monoclonal 抗体(mAb ) 。三 haptens (1 与 thiazole 包围的 hapten， 2 与苯包围的 hapten，和 3 与直碳锁住的 hapten ) 被用于抗原准备。与 hybridoma 技术，组特定的 mAb 与 50% 最大的禁止的集中被屏蔽(对 sulfathizole (STZ ) 和从 0.08 ～ 90.18 ng/mL 的另外的 25 类似物的 IC ₅₀) 。单音分散金 nanoparticles 与 mAb 被结合开发侧面的 immunochromatographic 长带。0.1 g/mL 的标记的抗体集中和在测试线的 0.2 g/mL 的涂层抗原集中为长带准备被选择。在优化条件，为 STZ 的集中的察觉( vLOD )的视觉限制， sulfamethoxazole ， sulfamethizole ，磺胺嘧啶， sulfamerazine ， sulfadimethoxine ， sulfamonomethoxine ， sulfameter ， sulfamethoxypyridazine ，和 sulfachloropyridazine 下面是 5 ， 0.25 ， 0.25 ， 10 ， 5 ， 10 ， 25 ， 2.5 ， 5 ， 0.25 ，和 10 g/kg 分别地。在蜂蜜样品的扫描仪分析为 26 硫安类药剂的察觉揭示了好性能。商业蜂蜜样品与传感器被测试，积极结果与高效的液体层析被证实。建议脱衣传感器在爱人样品为硫安类药剂污染物质的快速、可靠的决心提供一个方便方法。

英文摘要：

A gold immunochromatographic sensor （GICS） was developed for the rapid detection of 26 sulfonamides in honey samples. The sensor was based on a group-specific monoclonal antibody （mAb） that can recognize all 26 sulfonamides. Three haptens （hapten I with a thiazole ring, hapten 2 with a benzene ring, and hapten 3 with a straight carbon chain） were used for antigen preparation. With hybridoma technology, a group-specific mAb was screened with a 50% maximal inhibitory concentration （IC50） against sulfathizole （STZ） and the other 25 analogues ranging from 0.08 to 90.18 ng/mL. Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip. A labeled antibody concentration of 0.1 pg/mL and a coating antigen concentration of 0.2 μg/mL in the test line were chosen for strip preparation. Under optimized conditions, the visual limits of detection （vLOD） for the concentrations of STZ, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamerazine, sulfadimethoxine, sulfamonomethoxine, sulfameter, sulfamethoxypyridazine, and sulfachloropyridazine were 5, 0.25, 0.25, 10, 5, 10, 25, 2.5, 5, 0.25, and 10 μg/kg, respectively. Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides. Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography. The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples.