中文摘要：

为快速、同时的察觉(fluoro ) quinolones，认出 32 的广泛地特定的 monoclonal 抗体(mAb )(fluoro ) quinolone 抗菌素作为 hapten 用 norfloxacin 衍生物和 sarfloxacin 衍生物的混合物被准备。基于黄金， nanoparticles (AuNPs ) 当时是的 immunochromatographic 脱衣与山羊反老鼠抗体和抗原(联合到 ovalbumin 的 sarfloxacin ) 集合了，过去常分别地形成 C 线和 T 线。这抗原与竞争(fluoro ) 在与 mAbs 孵化的一件样品的 quinolones 用 AuNPs 标记。长带能检测 32 ( fluoro ) quinolones 包括 oxolinic 酸， nalidixic 酸， miloxacin ， pipemidic 酸， piromidic 酸， rosoxacin ， cinoxacin ， norfloxacin ， pefloxacin ， lomfloxacin ， enofloxacin ， fleroxacin ， ciprofloxacin ， enrofloxacin ， dafloxacin ， orbifloxacin ， sparfloxacin ， gemifloxacin ， besifloxacin ， balofloxacin ， gatifloxacin ，在在 10 mi 以内的牛奶的 moxifloxacin ， nadifloxacin ， ofloxacin ， marbofloxacin ， flumequine ， pazufloxacin ， prulifloxacin ， sarafloxacin ， difloxacin ， trovafloxacin ，和 tosufloxacin 从 1 ～ 100 ng/mL 和察觉的限制的长带范围的截止价值是 0.1-10 ng/mL。长带不包括四圜素， sulfamethazine，氨比西林，抗生素的一种，黄麴毒素 B1，或 gentamicin 与抗菌素做跨 react。简言之，这 immunochromatographic 脱衣是为主要屏蔽的一个很有用的工具(fluoro ) 在牛奶的 quinolones。

英文摘要：

For rapid and simultaneous detection of （fluoro）quinolones, a broadly specific monoclonal antibody （mAb） that recognizes 32 （fluoro）quinolone antibiotics was prepared using a mixture of a norfloxacin derivative and a sarfloxacin derivative as the hapten. An immunochromatographic strip based on gold nanoparticles （AuNPs） was then assembled with goat anti-mouse antibody and antigen （sarfloxacin coupled to ovalbumin）, used to form the C line and T line, respectively. This antigen competes with the （fluoro）quinolones in a sample incubated with mAbs labeled with AuNPs. The strip can detect 32 （fluoro）quinolones including oxolinic acid, nalidixic acid, miloxacin, pipemidic acid, piromidic acid, rosoxacin, cinoxacin, norfloxacin, pefloxacin, lomfloxacin, enofloxacin, fleroxacin, ciprofloxacin, enrofloxacin, dafloxacin, orbifloxacin, sparfloxacin, gemifloxacin, besifloxacin, balofloxacin, gatifloxacin, moxifloxacin, nadifloxacin, ofloxacin, marbofloxacin, flumequine, pazufloxacin, prulifloxacin, sarafloxacin, difloxacin, trovafloxacin, and tosufloxacin in milk within 10 min with the naked eye. The cut-off values of the strip range from 1 to 100 ng/mL and the limits of detection are 0.1- 10 ng/mL. The strip does not cross-react with antibiotics including tetracycline, sulfamethazine, ampicillin, erythromycin, aflatoxin B1, or gentamicin. In short, this immunochromatographic strip is a very useful tool for the primary screening of （fluoro）quinolones in milk.