设置60%和90%WHC两种土壤水分条件,并添加凋落物过滤液、剩余残渣和丙氨酸,进行为期36 d的室内培养(25℃),研究了凋落物中水溶性有机物和残渣对土壤氮素转化的影响.结果表明:在60%和90%WHC条件下,丙氨酸在土壤中迅速矿化,该处理的土壤铵态氮(NH_4^+-N)含量分别比对照显著提高5.4%~44.7%和16.1%~41.3%,净氮矿化和氨化速率在培养前期也高于对照,而凋落物过滤液和残渣添加处理则降低了土壤NH_4^+-N含量,且残渣的降幅大于过滤液.试验期间,土壤硝态氮(NO_3^--N)含量呈直线增长趋势,培养结束时60%WHC条件下NO_3--N含量显著高于90%WHC.土壤水分含量增多不利于土壤有机质的矿化;90%WHC条件下可溶性有机碳(SOC)含量明显低于60%WHC,而土壤氧化亚氮(N_2O)排放量比60%WHC提高1.5~63.0倍,且在60%WHC条件下凋落物残渣添加处理显著促进了土壤N_2O的排放.凋落物在分解过程中的可溶性物质和剩余物对土壤氮的影响存在差异,且这种差异随分解而发生动态变化.
An incubation experiment was carried out with the addition of litter filtrate, litter residue and alanine at 25 ℃ for 36 days under 60% and 90%WHC (water holding capacity) conditions. The results showed that alanine was rapidly mineralized in soil, and soil NHa+N content significant- ly increased by 5.4%-44.7% and 16.1%-41.3%, respectively under 60% and 90%WHC condi- tions compared with the control. The soil net nitrogen mineralization and ammonification rates in the two treatments were also higher than those in the control at the early stage of incubation. However, the soil NH4 ~-N content was reduced by the addition of filtrate and residue, and the reduction de- gree of residue was greater. During the incubation, soil NO3--N content showed a linear increasing trend with the incubation time, and it was significantly higher under the 60%WHC condition than that under 90%WHC condition at the end of incubation. The mineralization of soil organic matter was limited by higher soil moisture. Therefore, the soil soluble organic carbon (SOC) content under 90% WHC condition was obviously lower than that under 60%WHC, but nitrous oxide (N20) emis- sion was 1.5-63.0 times higher than that under 60%WHC. Furthermore, N20 emission was induced significantly by the addition of litter residue under 60%WHC condition. These results indicated that there were different effects of soluble matter and litter residue on soil nitrogen transformation, and these differences would change dynamically in the decomposition process.