中文摘要：

目的：探讨连翘苷对Lewis肺癌组织中血管内皮生长因子（VEGF）和血管生成抑制因子内皮抑制素（endostatin）蛋白表达的影响。方法：免疫组化染色检测临床收集的正常肺部组织、癌旁组织和肺癌组织样本中VEGF和endostatin的蛋白表达。设空白对照组10只,同时于40只小鼠右肢腋窝皮下接种Lewis瘤细胞,随机分为模型组、连翘苷低剂量组、连翘苷中剂量组和连翘苷高剂量组,每组10只。模型组每天1次灌胃等体积无菌水;连翘苷低、中剂量组每天灌胃5、10 g/kg连翘苷1次;高剂量组每天灌胃10 g/kg连翘苷2次。给药20 d后取肺部组织进行HE染色和免疫组化染色检测肿瘤组织形态学与肺部组织中VEGF和endostatin的蛋白表达。结果：临床样本中,与正常组织和癌旁组织相比,肺癌组织中的VEGF呈高表达,endostatin呈低表达。连翘苷随剂量增加可显著减少Lewis肺癌小鼠肿瘤体积和瘤组织密度;给予不同剂量连翘苷后,VEGF在肺癌组织中表达显著低于模型对照组,而endostatin表达则明显高于模型对照组。结论：连翘苷通过下调VEGF的表达、上调endostatin的表达而发挥抑制肺部肿瘤发展的作用。

英文摘要：

AIM： To investigate the effects of phillyrin on vascular endothelial growth factor（ VEGF） and endostatin expression in lung tumor tissues isolated from Lewis lung carcinoma. METHODS： The expression of VEGF and endostatin in control individuals and the patients with lung cancer was determined by immunohistochemistry. In the animal experiment,5 groups of animals were examined： control,tumor model,and tumor model with 3 different concentrations of phillyrin treatments. For preparation of transplanted tumor model,Lewis cells were subcutaneously injected into the right limb armpit of the nude mice. After that,phillyrin was administered via oral gavage once daily for 20 d at dose of 5 or 10 g / kg,or twice daily at 10 g / kg. Lung tumor tissues isolated from each group were observed by hematoxylin-eosin staining.VEGF and endostatin expression were examined by immunohistochemistry. RESULTS： VEGF expression was increased in lung tumor tissues as compared with normal and pericarcinous tissues,while endostatin expression was decreased. Phillyrin significantly inhibited the tumor size and tumor tissue density dose-dependently,which was accompanied with a decrease in VEGF expression and an increase in endostatin expression. CONCLUSION： Phillyrin inhibits the development of lung tumor through reducing VEGF expression and increasing endostatin expression.