中文摘要：

目的分析比较五种常见的上皮性卵巢癌细胞系IL-6、IL-8及其受体表达的差异。方法IL-6、IL-8的表达分别采用RT-PCR和ELISA法进行检测，IL-6受体（IL-6Rα和gp130）及IL-8受体（IL-8RA和IL-8RB）的表达采用免疫印迹技术进行测定。结果①五种上皮性卵巢癌细胞均组成性表达IL-6和IL-8。IL-6和IL-8在CAOV-3细胞中的表达水平均最高，而在HO-8910PM细胞中的表达水平均最低，IL-6在SKOV-3、HO-8910、OVCAR-3细胞中的表达水平依次降低，IL-8在OVCAR-3、SKOV-3、HO-8910细胞中的表达水平依次降低。②五种上皮性卵巢癌细胞均表达IL-6Rα、gp130及IL-8RA；除CAOV-3细胞外，其它细胞均表达IL-8RB。结论本研究旨在筛选表达IL-6和IL-8及其相应受体的细胞株，为研究IL-6、IL-8与卵巢癌发生、发展关系奠定基础，同时也为今后卵巢癌的免疫治疗提供一个新的思路。

英文摘要：

Objective To investigate the expression levels of interleukin 6 （IL-6）, interleukin 8 （IL-8）, and their receptors in five epithelial ovarian cancer cell lines. Methods Reverse transcription polymerase chain reaction （RT-PCR） and enzyme linked immunosorbent assay （ELISA） were performed to examine the expressions of IL-6 and IL-8, respectively. The expressions of IL-6 receptor （IL-6R） and IL-8 receptor （IL-8R） were detected by Western blot analysis. Results All epithelial ovarian cancer cell lines constitutively expressed IL-6 and IL-8. The expression levels of IL-6 and IL-8 in CAOV-3 cells were the highest, but in HO-8910PM cells were the lowest. The expression levels of IL-6 and IL-8 were cell line dependent. Five ovarian cancer cells all expressed IL-6R, gp130, and IL-8RA; five ovarian cancer cells except CAOV-3 cells expressed IL-8RB. Conclusion Data from this study could provide a basis for investigating the relationship of IL-6 and IL-8 with epithelial ovarian cancer, and is useful for designing new strategies of immunotherapy.