中文摘要：

目的：探讨Wnt／β-catenin通路在小鼠肾发育中的作用及差异表达基因。方法：采用基因芯片技术筛选小鼠肾发育过程中的差异表达基因；应用实时定量RT-PCR和免疫印迹观察不同胎龄小鼠肾组织中的β-cateninmRNA和活化的β-catenin蛋白表达水平进行半定量分析。应用免疫组织化学检测不同胚龄小鼠肾组织中活化的β-catenin蛋白表达的定位与定量。结果：pcateninmRNA的表达在不同胚龄小鼠肾组织中的表达是不同的。活化的β-catenin蛋白的表达量在E14．5d达到高峰，后逐渐下降。在出生后1d出现小高峰后，逐渐下降并维持在低水平。免疫荧光显示，在小鼠肾发生发育过程中，活化的β-catenin主要表达于输尿管芽及其分支和肾小管，。肾小球中表达量较少。结论：Wnt／β-catenin通路的时间和空间表达在肾发育中发挥重要的作用。

英文摘要：

Objective： To study the expression of Wnt/β-catenin signaling pathway during mouse kidney development and gene chip were used to screen differential expressed genes. Methods： Real-time RT-PCR and Western blotting were used to semi- quantitatively measure the expression of β-catenin mRNA and active β-catenin protein during development of mouse kidney. Immunohistochemical staining was used to examine the location of active β-catenin protein at different stages of embryonic and postnatal kidney. Results： Active β-catenin mRNA was expressed differently at different time points of kidney development. And the active β-catenin protein reached peak at El4. 5 d and then gradually decreased until the first day after birth, and then decreased gradually and maintained in low level 2 weeks after birth. Immunohistoehemical staining showed that the expression of active β-catenin was mainly expressed in the ureteric bud, its branch, and kidney tubules. And its expression was low in the glomerulus. Conclusion： The spatial and temporal expression of Wnt/β-catenin signaling pathway is essential during kidney development.