中文摘要：

目的探讨端粒酶在大鼠脊髓胶质瘢痕中的表达及其与脊髓胶质瘢痕形成的关系。方法将SD大鼠120只随机分为端粒酶未干扰组（未干扰组）、端粒酶干扰组（干扰组）及对照组，每组各40只。未干扰组、干扰组为脊髓损伤组，采用改良Allen’s重物坠落法造模；对照组只打开椎板，暴露脊髓，不造成脊髓损伤。每组于损伤后1、3、5、7、14、28、42、56d取损伤区域标本，用PCR—ELISA、Westernblot法检测胶质瘢痕中端粒酶、胶质酸性纤维蛋白（GFAP）的表达，并分析其相关性；免疫荧光观察胶质瘢痕的形成。结果未干扰组端粒酶在各时间点的表达量（0．180±0．004—1．217±0．072）明显高于干扰组（0．028±0．007～0．092±0．004，0=28．753～37．518，P〈0．05）和对照组（0．072±0．007—0．075±0．004，χ2=18．618～41．093，P〈0．05），差异有统计学意义。未干扰组GFAP在各时间点的表达量（1．98±0．15～19．40±0．55）明显高于干扰组（1．10±0．13～16．64±1．02，0=14．538—37．366，P〈0．05）和对照组（0．44±0．05—0．48±0．04，0=16．733—34．041，P〈0．05），且差异有统计学意义。未干扰组端粒酶的表达与GFAP表达呈相关线性关系且有统计学差异（r=0．755，P〈0．01）。干扰组和对照组端粒酶均呈阴性表达。免疫荧光观察未干扰组胶质瘢痕重于干扰组，对照组未见胶质瘢痕形成。结论端粒酶在脊髓胶质瘢痕中呈动态表达并与衡量胶质瘢痕程度的GFAP呈正相关线性关系，可能是促进胶质瘢痕形成的重要因素。

英文摘要：

Objective To detect the expression of telomerase in glial scar and its correlation with glial scar. Methods There were 120 Sprague Dawley rats were randomly divided into non-interference group of telomerase, interference group of telomerase and control group. Non-interference group and interference group were for spinal cord injury, which adopted Allen＇s Weight Dropping to make molding; control group was for sham operation to open the vertebral plate and expose spinal marrow, in which spinal cord injury would not be caused. The expression of telomerase and glial fibrillary acidic profein （GFAP） was detected by PCR-ELISA and Western blot, and the formation of glial scar was observed by immunofluoreseenee on the 1st, 3rd, 5th, 7th, 14th, 28th, 42th and 56th day after the spinal injury,and analyzed its relativity. Results The expression of telomerase in non-interference group was （0. 180 ± 0. 004-1. 217 ±0. 072）, which was significantly higher than those in interference group （0. 028 ± 0. 007- 0.092±0.004,χ2=28.753-37.518,P〈0.05） and control group （0.072 ±0.007-0.075 ±0.004,χ2 = 18. 618-41. 093 ,P 〈 0. 05 ） at all the time, with statistical significance. The expression of GFAP in noninterference group was （ 1.98 ± 0. 15-19. 40 -± 0. 55 ） which was significantly higher than those in interference group （ 1.10 ±0. 13-16. 64 ± 1.02,χ2 = 14. 538-37. 366,P 〈0. 05） and control group （0. 44 ±0. 05-0.48 ± 0. 04, χ2 = 16. 733-34. 041 ,P 〈 0.05 ） at all the time, with statistical significance. The expression of GFAP showed a linear correlation with that of telomerase in non-interference group, and with statistical differenees （r =0. 755, P 〈0. 01 ）. The expression of telomerase in interference group and control group were always negative. Glial scar observed by immunofluoreseence in non-interference group was heavier than that in interference group, and control group showed no formation of glial scar. Conclusions Telomerase shows a dynamic expression in glial scar and